1.13.11.34: arachidonate 5-lipoxygenase
This is an abbreviated version!
For detailed information about arachidonate 5-lipoxygenase, go to the full flat file.
Word Map on EC 1.13.11.34
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1.13.11.34
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cyclooxygenase
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prostaglandin
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leukocyte
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neutrophil
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eicosanoids
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cox-2
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asthma
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indomethacin
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ionophore
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phospholipase
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zileuton
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polymorphonuclear
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thromboxane
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flap
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airway
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peritoneal
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5-hete
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platelet
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eosinophil
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mast
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cysteinyl
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allergic
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edema
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12-lipoxygenase
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histamine
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lta4
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nordihydroguaiaretic
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lipoxygenases
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paf
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ndga
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bronchoconstriction
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5-hydroxyeicosatetraenoic
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asthmatic
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basophil
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platelet-activating
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anaphylaxis
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zymosan
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antigen-induced
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lipoxins
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montelukast
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hetes
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mpges-1
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carrageenan-induced
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a23187-stimulated
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paf-induced
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pro-resolving
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synthesis
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bronchospasm
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medicine
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drug development
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14carachidonic
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pharmacology
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bronchoconstrictors
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ulcerogenic
- 1.13.11.34
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cyclooxygenase
- prostaglandin
- leukocyte
- neutrophil
-
eicosanoids
- cox-2
- asthma
- indomethacin
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ionophore
- phospholipase
- zileuton
-
polymorphonuclear
-
thromboxane
- flap
- airway
- peritoneal
-
5-hete
- platelet
-
eosinophil
-
mast
-
cysteinyl
-
allergic
- edema
-
12-lipoxygenase
- histamine
- lta4
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nordihydroguaiaretic
- lipoxygenases
- paf
- ndga
-
bronchoconstriction
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5-hydroxyeicosatetraenoic
-
asthmatic
-
basophil
-
platelet-activating
- anaphylaxis
- zymosan
-
antigen-induced
-
lipoxins
- montelukast
-
hetes
- mpges-1
-
carrageenan-induced
-
a23187-stimulated
-
paf-induced
-
pro-resolving
- synthesis
- bronchospasm
- medicine
- drug development
-
14carachidonic
- pharmacology
-
bronchoconstrictors
-
ulcerogenic
Reaction
Synonyms
5-lipoxygenase, 5-LO, 5-LO1, 5-LOX, 5DELTA-lipoxygenase, 5LO, 5LOX-1, ALOX5, arachidonate 5-LO, arachidonate:oxygen oxidoreductase, arachidonic 5-lipoxygenase, arachidonic acid 5-lipoxygenase, C-5-lipoxygenase, DELTA5-lipoxygenase, H5-LO, leukotriene A4 synthase, leukotriene-A4 synthase, lipoxygenase 15, lipoxygenase 5, lipoxygenase-1, LO-1, LOX-15, LOX-5, LTA synthase, LTA4 synthase, oxygenase, arachidonate, 5-lip-, PMNL 5-lipoxygenase
ECTree
1.13.11.34 arachidonate 5-lipoxygenaseAdvanced search results
results (
results (Activating Compound
Activating Compound on EC 1.13.11.34 - arachidonate 5-lipoxygenase
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ACTIVATING COMPOUND 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
(13S)-hydroperoxy-9,11-octadecadienoyl lysophosphatidylcholine
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50% activation concentration AC50 is 0.0015 mM
1,25(OH)2D3
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gives a strong (100fold) upregulation of 5-lipoxygenase protein in differentiating Mono Mac 6 cells
5,8,11,14,17-eicosapentaenoic acid
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competitive inhibition of oxygenation of arachidonic acid
5-lipoxygenase activating protein
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5-lipoxygenase activating protein is crucial for conversion of endogenous substrate by 5LO 5-lipoxygenase activating protein also stimulates the utilization of exogenous arachidonic acid, and greatly (190fold) stimulates utilization of (5Z,8Z,10E,12S,14Z)-12-hydroxyicosa-5,8,10,14-tetraenoic acid
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5-LO activating protein
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i.e. FLAP, Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein
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guanosine 5'-O-(3-thiotriphosphate)
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10fold stimulation of arachidonic acid oxidation at 0.0005-0.001 mM
Nicotine
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treatment with 0.01 mM nicotine activates 5-lipoxygenase gastric cancer cell lines from 36-72 h
phosphatidyl choline
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addition increases catalytic activity of wild-type nzyme by 50%
phosphocholine
phosphocholine in combination with Ca2+ markedly stimulate the formation of leukotrienes by wild-type 5-LO and mutant C159S/C300S/C416S/C418S, whereas their effect on the 5-LO W13A/W75A/W102A mutant is small
transforming growth factor beta
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gives a strong (100fold) upregulation of 5-lipoxygenase protein in differentiating Mono Mac 6 cells
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5-lipoxygenase-activating protein
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functional 5-LOX requires binding to 5-lipoxygenase-activating protein which helps 5-LOX binding to arachidonic acid at the nuclear membrane and enhances the efficiency of leukotriene synthesis
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5-lipoxygenase-activating protein
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a 18000 Da integral membrane protein required, in peripheral cells, for the activation of 5-lipoxygenase
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5-LO-activating protein
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i.e. FLAP, a small permanently membrane-bound protein that is essential for the biosynthesis of leukotrienes from endogenous arachidonic acid. FLAP binds arachidonic acid and is thought to assist in the provision of substrate to 5-LO
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5-LO-activating protein
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i.e. FLAP, a small permanently membrane-bound protein that is essential for the biosynthesis of leukotrienes from endogenous arachidonic acid. FLAP binds arachidonic acid and is thought to assist in the provision of substrate to 5-LO
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ATP
activates, hyperbolic kinetic parameters for ATP activation indicate a similar activation for arachidonate and (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate. Solvent isotope effect results for both hydroperoxidation and epoxidation indicate that a specific step in the molecular mechanism is changed, possibly because of a lowering of the dependence of the rate-limiting step on hydrogen atom abstraction and an increase in the dependency on hydrogen bond rearrangement. Changes in ATP concentration in the cell can affect the production of 5-LOX products, such as leukotrienes and lipoxins, and thus have wide implications for the regulation of cellular inflammation
coactosin-like protein
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binds 5-LO and promotes leukotriene formation, coactosin-like protein prevents heat-inactivation of 5-LO
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FLAP
5-LO-activating protein, a small permanently membrane-bound protein that is essential for the biosynthesis of leukotrienes from endogenous arachidonic acid. FLAP binds arachidonic acid and is thought to assist in the provision of substrate to 5-LO
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FLAP
5-lipoxygenase-activating protein, exclusively located at the nuclear membrane
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FLAP
5-lipoxygenase-activating protein. Inhibitor BRP-7 blocks 5-LOX co-localization with FLAP at the nuclear envelope in neutrophils
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FLAP
the 5-LOX activating protein (FLAP) assists the translocation of 5-LOX from plasma membranes to nucleus upon cell stimulation
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phosphatidylcholine
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enzyme activity is supported by phosphatidylcholine more than by any other lipid tested, except for a cationic lipid, which is more stimulatory than phosphatidylcholine
protein factors from human leukocyte
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characterization of the membrane-associated stimulating factor
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additional information
coactosin-like protein, i.e. CLP, can bind to 5-LO and supports Ca2+-induced 5-LO enzyme activity. CLP seems to function as a chaperone or scaffold for 5-LO. About 100fold induction of 5-LO mRNA, protein and activity is found after differentiation of HL-60 and human monocytic Mono Mac 6 cells with TGF-beta and 1,25(OH)2D3, upregulation of 5-LO through the C2-like domain. Factors like cell stress and phorbol ester activate MAPK kinases, which phosphorylate the enzyme and enhance its activity and induce nuclear translocation, overview
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additional information
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the enzym eis upregulated in Epstein-Barr virus infected cells
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additional information
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calcium ionophore A23187 increases concentrations of 5(S)-hydroxy-6,8,11,14-(E,Z,Z,Z)-eicosatetraenoic acid from 0.07 to 45.5 pmol/10000000 cells
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additional information
the enzyme mutant 5-LODELTA4 stimulates wild-type 5-LO activity and product formation at low protein concentrations
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additional information
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the enzyme mutant 5-LODELTA4 stimulates wild-type 5-LO activity and product formation at low protein concentrations
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additional information
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coactosin-like protein, i.e. CLP, can bind to 5-LO and supports Ca2+-induced 5-LO enzyme activity. CLP seems to function as a chaperone or scaffold for 5-LO, upregulation of 5-LO through the C2-like domain
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additional information
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in the mouse 5-lipoxygenase gene there are no GC-boxes in tandem, the core promoter contains only one Sp1/3 binding site
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additional information
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coactosin-like protein, i.e. CLP, can bind to 5-LO and supports Ca2+-induced 5-LO enzyme activity. CLP seems to function as a chaperone or scaffold for 5-LO, upregulation of 5-LO through the C2-like domain
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