1.13.11.15: 3,4-dihydroxyphenylacetate 2,3-dioxygenase
This is an abbreviated version!
For detailed information about 3,4-dihydroxyphenylacetate 2,3-dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.15
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1.13.11.15
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extradiol
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catecholate
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4-nitrocatechol
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fuscum
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brevibacterium
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globiformis
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manganese-dependent
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ring-cleaving
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extradiol-cleaving
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second-sphere
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monoanionic
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hydroperoxo
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4-hydroxyphenylacetate
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superoxo
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alkylperoxo
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feiii-superoxo
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manganeseii
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ortho-dihydroxylated
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side-on
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crystallo
- 1.13.11.15
-
extradiol
-
catecholate
- 4-nitrocatechol
- fuscum
-
brevibacterium
- globiformis
-
manganese-dependent
-
ring-cleaving
-
extradiol-cleaving
-
second-sphere
-
monoanionic
-
hydroperoxo
- 4-hydroxyphenylacetate
-
superoxo
-
alkylperoxo
-
feiii-superoxo
-
manganeseii
-
ortho-dihydroxylated
-
side-on
-
crystallo
Reaction
Synonyms
2,3-HPCD, 3,4-dihydroxyphenylacetic acid 2,3-dioxygenase, Bf 2,3-HPCD, DHPAO, Fe-HPCD, Fe-MndD, FeHPCD, homoprotocatechuate 2,3 dioxygenase, homoprotocatechuate 2,3-dioxygenase, homoprotocatechuate dioxygenase, HPADO, HPC 2,3-dioxygenase, HPC dioxygenase, HPCA 2,3-dioxygenase, HPCD, Mn(II)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase, Mn-HPCD, Mn-MndD, MndD, MnHPCD, oxygenase, homoprotocatechuate 2,3-di-, PaDHPAO
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Purification
Purification on EC 1.13.11.15 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase
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partial
recombinant soluble enzyme 2.5fold from Escherichia coli strain BL21 (DE3) by anion exchange chromatography, ultrafiltration, hydrophobic interaction chromatography, ultrafiltration, and gel filtration
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recombinant wild-type and mutant Y257F enzymes by a procedure including anion exchange chromatography
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