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1.13.11.15: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

This is an abbreviated version!
For detailed information about 3,4-dihydroxyphenylacetate 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.15

Reaction

3,4-Dihydroxyphenylacetate
+
O2
=
2-hydroxy-5-carboxymethylmuconate semialdehyde

Synonyms

2,3-HPCD, 3,4-dihydroxyphenylacetic acid 2,3-dioxygenase, Bf 2,3-HPCD, DHPAO, Fe-HPCD, Fe-MndD, FeHPCD, homoprotocatechuate 2,3 dioxygenase, homoprotocatechuate 2,3-dioxygenase, homoprotocatechuate dioxygenase, HPADO, HPC 2,3-dioxygenase, HPC dioxygenase, HPCA 2,3-dioxygenase, HPCD, Mn(II)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase, Mn-HPCD, Mn-MndD, MndD, MnHPCD, oxygenase, homoprotocatechuate 2,3-di-, PaDHPAO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.15 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Purification

Purification on EC 1.13.11.15 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial
recombinant enzyme from Escherichia coli
-
recombinant soluble enzyme 2.5fold from Escherichia coli strain BL21 (DE3) by anion exchange chromatography, ultrafiltration, hydrophobic interaction chromatography, ultrafiltration, and gel filtration
-
recombinant wild-type and mutant Y257F enzymes by a procedure including anion exchange chromatography
-
wild-type and mutant enzymes
-
wild-type enzyme and H200 mutants
-