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1.13.11.15: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

This is an abbreviated version!
For detailed information about 3,4-dihydroxyphenylacetate 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.15

Reaction

3,4-Dihydroxyphenylacetate
+
O2
=
2-hydroxy-5-carboxymethylmuconate semialdehyde

Synonyms

2,3-HPCD, 3,4-dihydroxyphenylacetic acid 2,3-dioxygenase, Bf 2,3-HPCD, DHPAO, Fe-HPCD, Fe-MndD, FeHPCD, homoprotocatechuate 2,3 dioxygenase, homoprotocatechuate 2,3-dioxygenase, homoprotocatechuate dioxygenase, HPADO, HPC 2,3-dioxygenase, HPC dioxygenase, HPCA 2,3-dioxygenase, HPCD, Mn(II)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase, Mn-HPCD, Mn-MndD, MndD, MnHPCD, oxygenase, homoprotocatechuate 2,3-di-, PaDHPAO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.15 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Inhibitors

Inhibitors on EC 1.13.11.15 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
3,4-dichlorophenylacetate
-
5 mM, 50% inhibition
3,4-dihydroxybenzoate
3,4-dihydroxycinnamate
-
-
3,4-Dihydroxyphenylpropionate
-
-
4-methylcatechol
-
-
4-nitrocatechol
5,5'-dithiobis-(2-nitrobenzoate)
Arthrobacter synephrinum
-
1 mM, complete inhibition
8-hydroxyquinoline
air
-
initially high activity of Fe-MndD, becomes inactivtated within one h
-
Copper salicylate
-
0.166 mM, rate of oxygen uptake is decreased by 38%, rate of product formation is diminished by 58%
Cu2+
-
0.01 mM, 80% inhibition
dithiothreitol
-
0.1 mM, 13% inhibition
DTT
-
destabilizes the enzyme
EDTA
-
destabilizes the enzyme
ferricyanide
Ferrous ammonium sulfate
-
0.1 mM, 32% inhibition
K3Fe(CN)6
-
10 mM, 35% inhibition
m-hydroxyphenylacetate
-
-
Mn2+
-
slight inhibition
Na2SO4
-
destabilizes the enzyme
NaN3
-
slightly destabilizes the enzyme
p-hydroxymercuribenzoate
-
0.1 mM, 13% inhibition
p-hydroxyphenylacetate
-
-
succinate semialdehyde
-
5 mM 65% inhibition
additional information
-