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1.13.11.12: linoleate 13S-lipoxygenase

This is an abbreviated version!
For detailed information about linoleate 13S-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.12

Reaction

alpha-linolenate
+
O2
=
(9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate

Synonyms

(13S)-lipoxygenase, 13-lipoxygenase, 13-LOX, 13S-lipoxygenase, 15-lipoxygenase-1, 15-LOX-1, 9/13-LOX, acidic lipoxygenase, Alox15, arachidonic acid 15S-lipoxygenating enzyme, AtLOX-2, AtLOX-3, AtLOX-4, AtLOX-6, CmLOX10, CmLOX13, iron 13S-lipoxygenase, liipoxygenase, linoleate 13-lipoxygenase, linoleate 13S-lipoxygenase, linoleate oxygen oxidoreductase, linoleate: oxygen oxidoreductase, linoleate:oxygen oxidoreductase, lipoxygenase, lipoxygenase 2, lipoxygenase-1, lipoxygenase-2, lipoxygenase-3, lipoxygenase-4, lipoxygenase-6, LO-1, LOX, LOX-1, LOX-2, LOX1, LOX10, LOX11, LOX12, LOX2, LOX2:Hv:1, LOX2:Md:2b, LOX2b, LOX3, LOX4, LOX6, LOX7, LOX8, LOX9, LoxA, Lpx-A2, MSD2, Oep1LOX2, Oep2LOX2, omega-6 linoleate lipoxygenase, PA-LOX, RCI-1, SBLO-1, secreted lipoxygenase, SLO, soybean lipoxygenase-1, StLOX2, StLOX3, type-1 LOX, type-2 13-LOX

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.12 linoleate 13S-lipoxygenase

Specific Activity

Specific Activity on EC 1.13.11.12 - linoleate 13S-lipoxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.04
-
crude enzyme, at pH 9.5 and 40°C
10.2
-
crude extract, with linoleic acid as substrate
10.5
-
purified His-tagged enzyme, pH 7.5, 25°C, with substrate alpha-linolenic acid
12
-
crude extract, with alpha-linolenic acid as substrate
15733.3
-
purified native enzyme, pH 6.0, 40°C
2.9
-
purified His-tagged enzyme, pH 7.5, 25°C, with substrate gamma-linolenic acid
22.6
-
purified His-tagged enzyme, pH 7.5, 25°C, with substrate arachidonic acid
23.4
26fold purified enzyme, with linoleic acid as substrate
26.4
-
purified His-tagged enzyme, pH 7.5, 25°C, with substrate linoleic acid
28.3
-
purified recombinant enzyme, pH 7.5, 25°C
3
-
after 85fold purification, at pH 9.5 and 40°C
474
-
55fold purified enzyme, with alpha-linolenic acid as substrate
5538
-
purified native enzyme, pH 6.0, 40°C
564
-
55fold purified enzyme, with linoleic acid as substrate