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1.12.1.2: hydrogen dehydrogenase

This is an abbreviated version!
For detailed information about hydrogen dehydrogenase, go to the full flat file.

Word Map on EC 1.12.1.2

Reaction

H2
+
NAD+
=
H+
+
NADH

Synonyms

bidirectional hydrogenase, bidirectional Ni-Fe hydrogenase, bidirectional NiFe-hydrogenase, bidirectional [NiFe] hydrogenase, dehydrogenase, hydrogen, FeHyd, H2:NAD+ oxidoreductase, H2ase, Hox, Hox hydrogenase, Hox type hydrogenase, HoxE, HoxEFUYH, HoxF, HoxFUYHI2, HoxH, HoxS, HoxU, HoxY, hydrogen-evolving enzyme, hydrogenase, Hyn, iron-hydrogenase, NAD+-reducing hydrogenase, NAD+-reducing NiFe hydrogenase, NAD+-reducing [NiFe]-hydrogenase, NAD-dependent hydrogenase, NAD-reducing hydrogenase, NADH-dependent Fe-only hydrogenase, NiFe hydrogenase, NiFe-type reversible hydrogenase, SH, soluble hydrogenase, soluble [NiFe]-hydrogenase, [FeFe] hydrogenase, [FeFe]-hydrogenase, [NiFe] H2ase, [NiFe] hydrogenase, [NiFe]-hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.1 With NAD+ or NADP+ as acceptor
                1.12.1.2 hydrogen dehydrogenase

Molecular Weight

Molecular Weight on EC 1.12.1.2 - hydrogen dehydrogenase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
106000
-
non-denaturing PAGE
14000
-
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE
162500
-
amino acid sequence calculation
178000
-
sucrose density gradient centrifugation
18000
x * 18000, HoxE, SDS-PAGE, x * 21000, recombinant HIs-tagged HoxE, SDS-PAGE
20000
-
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE
200000
-
gel filtration
204500
-
gel filtration
206000
-
sucrose density gradient centrifugation
21000
x * 18000, HoxE, SDS-PAGE, x * 21000, recombinant HIs-tagged HoxE, SDS-PAGE
23000
-
1 * 67000 + 1 * 55000 + 1 * 26000 + 1 * 23000
26000
27000
-
1 * 64000 + 1 * 56000 + 1 * 31000 + 1 * 27000, native hydrogenase dissociates into two subunit dimers of 64000 Da and 1 * 31000 Da, and of 56000 Da and 27000 Da respectively, SDS-PAGE
30000
-
x * 63000 + x * 30000 + x * 26000 + x * 56000, SDS-PAGE, deduced from nucleotide sequence
31000
-
1 * 64000 + 1 * 56000 + 1 * 31000 + 1 * 27000, native hydrogenase dissociates into two subunit dimers of 64000 Da and 1 * 31000 Da, and of 56000 Da and 27000 Da respectively, SDS-PAGE
375000
native dimeric assembly of the pentameric enzyme complex, gel filtration
55000
-
1 * 67000 + 1 * 55000 + 1 * 26000 + 1 * 23000
56000
63000
-
x * 63000 + x * 30000 + x * 26000 + x * 56000, SDS-PAGE, deduced from nucleotide sequence
64000
65000
-
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE
67000
-
1 * 67000 + 1 * 55000 + 1 * 26000 + 1 * 23000