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4Fe-4S-center
presence of a 4Fe-4S-center in addition to the active site iron
benzyl viologen
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oxidized and reduced
Ferredoxin
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the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically for catalysis of H2 production
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NAD(P)H
NADH is 2times more active than NADPH
[2Fe-2S]-center
HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters
[4Fe-4S]-center
HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters
flavin
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flavoprotein
flavin
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only when FMN or riboflavin are added in combination with Mg2+ or Ni2+, NAD-reducing system is activated
flavin
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1 molecule of FMN per enzyme molecule
flavin
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2 molecules of FMN per enzyme molecule
FMN
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FMN
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enzyme contains FMN
FMN
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2 FMN molecules per enzyme molecule
FMN
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1 FMN molecule per enzyme molecule
FMN
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2 molecules per enzyme molecule, one of 2 can be reversibly released upon reduction of the enzyme
FMN
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bound to the diaphorase subunits
FMN
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required, 2 molecules per tetrameric enzyme complex, reduced enzyme reversibly releases half of the FMN bound, kinetics, FMN release induces reduction with NADH, overview
FMN
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required, enzyme contains 1 mol FMN per mol of enzyme
FMN
cofactor and stabilization of the active site
FMN
module HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters
iron-sulfur centre
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iron-sulfur centre
Solidesulfovibrio fructosivorans
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NAD+
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NAD+
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highly specific for
NAD+
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dependent on, binding site on the diaphorase subunits
NADH
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NADH
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highly specific for
NADH
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dependent on, binding site on the diaphorase subunits
NADH
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the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically for catalysis of H2 production
additional information
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no activity with NADP+
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additional information
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benzyl viologen, methyl viologen or methylene blue can replace NAD+/NADH
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additional information
methyl viologen can replace NAD+/NADH
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additional information
methyl viologen can replace NAD+/NADH
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additional information
methyl viologen can replace NAD+/NADH
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additional information
methyl viologen can replace NAD+/NADH
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additional information
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methyl viologen or benzyl viologen can replace NAD+/NADH
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additional information
methyl viologen or dithionite can act as electron donor/acceptor
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additional information
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methyl viologen, oxidized and reduced can replace NAD+/NADH
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additional information
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Ni-Fe cofactor, analysis of the non-standard structure of the Ni-Fe cofactor bound at the active site, interaction mechanism with H2, structural changes during reaction, reduction, and inactivation, the cofactor structure is (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO), overview
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additional information
no activity with ferredoxin or FMN
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additional information
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no activity with NADPH and NADP+
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additional information
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no activity with NADPH/NADP+
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