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1.12.1.2: hydrogen dehydrogenase

This is an abbreviated version!
For detailed information about hydrogen dehydrogenase, go to the full flat file.

Word Map on EC 1.12.1.2

Reaction

H2
+
NAD+
=
H+
+
NADH

Synonyms

bidirectional hydrogenase, bidirectional Ni-Fe hydrogenase, bidirectional NiFe-hydrogenase, bidirectional [NiFe] hydrogenase, dehydrogenase, hydrogen, FeHyd, H2:NAD+ oxidoreductase, H2ase, Hox, Hox hydrogenase, Hox type hydrogenase, HoxE, HoxEFUYH, HoxF, HoxFUYHI2, HoxH, HoxS, HoxU, HoxY, hydrogen-evolving enzyme, hydrogenase, Hyn, iron-hydrogenase, NAD+-reducing hydrogenase, NAD+-reducing NiFe hydrogenase, NAD+-reducing [NiFe]-hydrogenase, NAD-dependent hydrogenase, NAD-reducing hydrogenase, NADH-dependent Fe-only hydrogenase, NiFe hydrogenase, NiFe-type reversible hydrogenase, SH, soluble hydrogenase, soluble [NiFe]-hydrogenase, [FeFe] hydrogenase, [FeFe]-hydrogenase, [NiFe] H2ase, [NiFe] hydrogenase, [NiFe]-hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.1 With NAD+ or NADP+ as acceptor
                1.12.1.2 hydrogen dehydrogenase

Cloned

Cloned on EC 1.12.1.2 - hydrogen dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, HoxE is encoded in a cluster of 5 genes encoding the enzyme complex subunits, sigma54-type promotor with probably inducible expression of the operon
expression in Cupriavidus necator
gene cluster HoxFUYHI, cloning and expression of the Strep-tagged HoxI-deletion mutant, a tetramer composed of HoxFU and HoxHY dimers forming a tetramer in Escherichia coli strains JM109 and XL-1 Blue
-
heterologous expression in Ralstonia eutropha
-
over-expression of a plasmid carrying the hoxFUYWI-hypA2B2F2CDEXA genes in Cupriavidus necator
-
overexpression of HoxE in Escherichia coli strain M15