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1.11.1.B2: chloride peroxidase (vanadium-containing)

This is an abbreviated version!
For detailed information about chloride peroxidase (vanadium-containing), go to the full flat file.

Word Map on EC 1.11.1.B2

Reaction

RH
+
Cl-
+
H2O2
+
H+
=
RCl
+ 2 H2O

Synonyms

chloroperoxidase, CPO, Mcl24, More, NapH1, PPHY, V-containing-haloperoxidase, vanadium chloroperoxidase, vanadium haloperoxidase, vanadium-containing chloroperoxidase, vanadium-containing peroxidase, vanadium-dependent chloroperoxidase, vanadium-dependent haloperoxidase, vCPO, VHPO

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.B2 chloride peroxidase (vanadium-containing)

Purification

Purification on EC 1.11.1.B2 - chloride peroxidase (vanadium-containing)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant CPO and mutant enzymes S402A and F397H are overexpressed as apoenzyme in Saccharomyces cerevisiae
recombinant enzyme
-
recombinant extracellular enzyme from Pichia pastoris strain X33 cell culture medium by lyophilization, anion exchange chromatography, and gel filtration, to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) by nickel affinity chromatography and alternating steps of ultrafiltration and desalting gel filtration, method overview