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1.11.1.6: catalase

This is an abbreviated version!
For detailed information about catalase, go to the full flat file.

Word Map on EC 1.11.1.6

Reaction

2 H2O2 =

O2
+ 2 H2O

Synonyms

Ab-catalase, BNC, caperase, CAT, CAT-1, CAT-A, CAT-P, Cat1.4, CatA, catalase, catalase A, catalase C, catalase form III, catalase P, catalase-1, catalase-A, catalase-peroxidase, catalase-phenol oxidase, CatB, CATC, CatF, CatG, CatP, CATPO, CcmC, CP, equilase, H2O2:H2O2 oxidoreductase, haem catalase, HPI-A, HPI-B, HPII, HTHP, hydrogen peroxide oxidoreductase, KAT, Kat E catalase, KatA, KatB, KatC, KatP, KpA, manganese catalase, More, optidase, PktA, polyethylene glycol-catalase, tyrosine-coordinated heme protein, VktA

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.6 catalase

General Stability

General Stability on EC 1.11.1.6 - catalase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is still active in 0.1% SDS
-
enzyme shows a high resistance to denaturing agents like 10% 2-mercaptoethanol
enzyme stability at 23°C, 37°C or 50°C in nonionic micellar and reverse micellar systems, formed by mixing of Brij 30, Brij 35, cyclohexane, decaline, dodecane, n-heptane or isooctane, and water
-
free and immobilized catalases show their maximum activities at 50 mM buffer concentration. When the buffer concentration is increased from 25 to 100 mM, the activity of Eupergit C-immobilized catalase is more affected than the activity of free catalase. At 100 mM buffer concentration, free catalase retains 96.9% of its maximum activity although immobilized catalase retains 80% of its maximum activity
-
incubation in the light clearly inhibits the activity of catalase, about 40% of the activity is lost within 2 h and only 25% remain after 5 h of incubation in the light
-
iron-bound [FeII/FeII]-ADE undergoes more than 100 turnovers with H2O2 before the enzyme is inactivated due to oxygenation of histidine residues critical for metal binding
-
isoform Cat-1, very stable for months, resistant to denaturizing agents such as urea or guanidine-HCl
NaCl decreases stability, ethanol, glycerol, bovine serum albumin enhance stabilitiy
-
polyethylene glycol and glycol stabilize best, 3-7fold increase in enzyme stability at 30°C, pH 7.0
-
propane-1,2,3-triol is protective against thermoinactivation of the enzyme at 43°C, but less at 55°C
-
protein must be purified in the presence of 2 M NaCl, although its highest activity is observed in the absence of salt
-
resistant to urea, 2% SDS or reducing agents
-
resistant up to 7 M urea, 10% SDS
-
sucrose is protective against thermoinactivation of the enzyme at 43°C and 55°C
-