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1.11.1.13: manganese peroxidase

This is an abbreviated version!
For detailed information about manganese peroxidase, go to the full flat file.

Word Map on EC 1.11.1.13

Reaction

2 Mn(II) + 2 H+ +

H2O2
= 2 Mn(III) + 2 H2O

Synonyms

CmMnP, extralong PMnP, hybrid Mn-peroxidase, Il-MnP1, Il-MnP6, L-MnP, LeMnP2, long PMnP, manganese peroxidase, manganese peroxidase 2, manganese-dependent peroxidase, MGmn2, MGmnp1, MGmnp3, Mn-dependent (NADH-oxidizing) peroxidase, Mn2+: hydrogen peroxide oxidoreductase, Mn2+:H2O2 oxidoreductase, Mn2+:hydrogen peroxide oxidoreductase, MnP, MnP 1, MnP II, MnP-BBP6, MnP-GY, MnP-PGY, mnp1, MnP10, MnP117436, MnP12, MnP157986, MnP2, MnP3, MnP50297, MnP6, Moror_3885, MP, MrMnP1, multifunctional manganese peroxidase, Nf b19 MNP2, peroxidase, manganese, peroxidase-M2, rMnP3-BBP6, short manganese peroxidase, short MnP

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.13 manganese peroxidase

Purification

Purification on EC 1.11.1.13 - manganese peroxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
11.1fold purification
-
12fold purification
-
148fold purification of MnP1 and 157fold purification of MnP2
-
2 isoenzymes: BOS1 and BOS2
-
24fold purification
-
25fold purification
-
3 isoenzymes
-
35.8fold purification
-
8.1-16fold purification
-
affinity chromatography on blue agarose: isozyme H4 95% pure, followed by preparative isoelectric focusing: isozymes H3 and H5 pure
-
enzymes are extracted with 50 mM citrate-phosphate buffer at pH 4.0, 5.0 and 6.0 at 10°C and 25°C
-
extract is loaded onto a HiPREP26/10 desalting column followd by chromatography on a DEAE-Sepharose and a MonoQ HR5/5 column, finally the active fractions are applied to CIM QA Disks
-
from Ceriporiopsis subvermispora by gel filtration and chromatography on a Mono-Q column
-
from stem juice
-
isoenzymes H4 and H5
-
MnP1 and MnP2
MnP1: 19fold purification
-
MnP2 and MnP3, purified from poplar culture
-
MnPI, MnPII and MnPIII
-
native enzyme 2.73fold from strain NITW715076, 1.6fold from strain NITW715076_1, and 2.85fold from strain NITW715076_2, by ammonium sulfate fractionation, dialysis, and anion exchange chromatography
-
native enzyme 6.9fold by anion exchange chromatography
native enzyme from cell free supernatant of bacterial culture, followed by ultrafiltration and gel filtration
-
partial purification
partial purification, MnP2 and MnP3 are not separated
-
protoplasts from mycelia, native and recombinant extracellular enzyme from mycelia by filtration, addition of PEG, and anion exchange chromatography (elution with succinate), and ultrafiltration
purification of a protein complex containing MnP, laccase and beta-glucosidase activities
-
purification of MnPs from the mutants F190Y, F190L, F190I and F190A
-
purification of recombinant A48C/A63C double mutant MnP, expressed in Escherichia coli
-
purification of recombinant MnP, expressed in Escherichia coli
purification of recombinant MnP, expressed in Phanerochaete chrysosporium
-
purification of the mutant enzymes R177D, R177E, R177N and R177Q
-
purification scheme involves ultrafiltration, affinity chromatography on concanavalin-A Sepharose and gel filtration
-
recombinant refolded His6-tagged enzyme expressed from Escherichia coli strain Rosetta (DE3) by dialysis and nickel affinity chromatography
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) in presence of 1 mM CaCl2 by nickel affinity chromatography, dialysis, followed by anion exchange chromatography, ultrafiltration, and gel filtration. Heme incorporation into MnP after purification, and removal of excess heme by high speed centrifugation at 50000 x g
ultrafiltration, Concanavalin-A Sepharose column chromatography and Superdex 75 gel filtration
-
using a Toyopearl Butyl-650M, a Toyopearl DEAE-650 and a Superdex 75 HR 10/30 column