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1.11.1.12: phospholipid-hydroperoxide glutathione peroxidase

This is an abbreviated version!
For detailed information about phospholipid-hydroperoxide glutathione peroxidase, go to the full flat file.

Word Map on EC 1.11.1.12

Reaction

2 glutathione +

a hydroperoxy-fatty-acyl-[lipid]
=
glutathione disulfide
 +
a hydroxy-fatty-acyl-[lipid]
 +
H2O

Synonyms

AC-PHGPx, Bi-PHGPx, class 4 GPx, CsGPx1, CsGPx2, CsGPx3, CsGPx4, DMPHGPx, glutathione peroxidase 4, glutathione peroxidase-4, glutathione-dependent phospholipid peroxidase, GPX, GPx-4, GPX3, GPx4, GPx41, GPx4A, GPx4B, GPXhs2, GPXle1, hydroperoxide glutathione peroxidase, Hyr1, Hyr1/YIR037W, McPHGPx, More, non-selenocysteine PHGPx, NPGPx, nPHGPx, OsPHGPx, peroxidation-inhibiting protein, peroxidation-inhibiting protein: peroxidase, glutathione (phospholipid hydroperoxide-reducing), PHGPX, phospholipid glutathione peroxidase, phospholipid hydroperoxidase, phospholipid hydroperoxide glutathione peroxidase, phospholipid hydroperoxide glutathione peroxidase A, phospholipid hydroperoxide glutathione peroxidase B, phospholipid hydroperoxide glutathione peroxidase-4, phospholipid-hydroperoxide glutathione peroxidase, selenium-dependent glutathione peroxidase type-4, seleno GPx, selenoperoxidase, selenoprotein P, sperm nucleus-specific glutathione peroxidase, TTHERM_00661720, type-4 glutathione peroxidase, VcPHGPx

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.12 phospholipid-hydroperoxide glutathione peroxidase

Cloned

Cloned on EC 1.11.1.12 - phospholipid-hydroperoxide glutathione peroxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in MIA PaCa-2 and AsPC-1 human pancreatic cancer cells
-
expressed in tumor cell transfectant clone 7G4
-
expression in a cysteine-auxotrophic Escherichia coli strain
expression in Escherichia coli
expression in HEK293-6E cell
expression of mutant enzyme U46C in Escherichia coli
expression of wild-type enzyme and SeC46C mutant in baculovirus/insect cell system and expression of mutant SeC46C as His-tagged fusion protein in Escherichia coli
-
expression vector encoding mitochondrial enzyme for overexpression in (RBL)2H3 cell line
-
expresssion in HEK-293T cell
for sequencing
-
gene Gpx4, quantitative real-time PCR expression analysis and absolute quantification of GPx4, recombinant overexpression of hGPx4 in insulin-producing RINm5F cells and in INS-1E cells
gene KfPHGPx, DNA and amino acid sequence determination and analysis, reverse transcription PCR expression analysis, the KfPHGPx expression patterns are analysed using semi-quantitative RT-PCR and real-time PCR, sequence comparisons and phylogenetic tree
-
His-tagged protein termed TcGPX1, expression in Escherichia coli
-
into the vector pET-28a+ for expression in Escherichia coli BL21DE3 cells, into the vector YEpGAP112 for a functional complementation test in yeast
into the vector pYEX-S1 for transformation into Saccharomyces cerevisiae cells
production of a transgenic mouse line overexpressing mPHGPx in the testis during the prophase of the first meiotic division, when the endogenous mPHGPx level is markedly lower than in the postmeiotic phase. Such mPHGPx overexpression is associated with male germ apoptosis, seminiferous tubule disorganization and reduced fertility
recombinant enzyme expression in HEK-293T cells
spermatozoa enzyme, expression in Escherichia coli as glutathione-S-transferase fusion protein
-
the baculovirus expression vector system, using the Autographa californica nucleopolyhedrovirus and the Sf9 insect cell line, is used to produce recombinant Bi-PHGPx proteins, the cDNA fragment containing the full-length open reading frame is inserted into the transfer vector pBAC1
the codon optimized gene is cloned into the vector pGex4T-1 for expression in Escherichia coli BL21DE3 cells
-
the entire encoding region for Oryza sativa PHGPx is expressed in Escherichia coli M15
-
the open reading frame of Hyr1/YIR037W is cloned into a pET28a-derived vector for expression in Escherichia coli Rosetta DE3 cells
-
the triple deletion mutant gpx1delta/gpx2delta/gpx3delta is not growing with medium containing unsaturated linolenate
-
transgenic mice overexpressing human GPx4 are generated
-
wild-type enzyme gpx1, gpx2, gpx3 and mutants, expression in Escherichia coli
-