1.10.3.2: laccase
This is an abbreviated version!
For detailed information about laccase, go to the full flat file.
Word Map on EC 1.10.3.2
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1.10.3.2
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trametes
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decolor
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versicolor
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abts
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white-rot
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manganese
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basidiomycete
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pleurotus
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oxidases
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wastewater
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textile
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effluent
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ostreatus
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bioremediation
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electrode
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lignocellulosic
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recalcitrant
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pulp
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guaiacol
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straw
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wood
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tyrosinase
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mushroom
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solid-state
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submerged
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cellulase
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xylanase
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brilliant
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phanerochaete
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chemoradiation
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chrysosporium
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malachite
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trinuclear
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veratryl
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kraft
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chemoradiotherapy
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copper-containing
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reusability
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monophenols
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indigo
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edodes
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humic
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bagasse
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brachytherapy
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bioelectrocatalytic
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copper-binding
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azoreductase
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monolignols
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industry
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para-aortic
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food industry
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degradation
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biofuel production
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energy production
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medicine
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environmental protection
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biotechnology
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analysis
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cmcase
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synthesis
- 1.10.3.2
- trametes
-
decolor
- versicolor
- abts
-
white-rot
- manganese
-
basidiomycete
- pleurotus
- oxidases
-
wastewater
-
textile
-
effluent
- ostreatus
-
bioremediation
-
electrode
-
lignocellulosic
-
recalcitrant
- pulp
- guaiacol
- straw
- wood
- tyrosinase
- mushroom
-
solid-state
-
submerged
- cellulase
- xylanase
-
brilliant
- phanerochaete
-
chemoradiation
- chrysosporium
- malachite
-
trinuclear
-
veratryl
-
kraft
-
chemoradiotherapy
-
copper-containing
-
reusability
- monophenols
- indigo
- edodes
-
humic
- bagasse
-
brachytherapy
-
bioelectrocatalytic
-
copper-binding
- azoreductase
- monolignols
- industry
-
para-aortic
- food industry
- degradation
- biofuel production
- energy production
- medicine
- environmental protection
- biotechnology
- analysis
- cmcase
- synthesis
Reaction
4 benzenediol
+
Synonyms
ATM, benzenediol oxygen oxidoreductase, benzenediol-oxygen oxidoreductase, benzenediol: oxygen oxidoreductase, benzenediol:O2 oxidoreductase, Benzenediol:oxygen oxidoreductase, blue laccase, blue multicopper oxidase, CcLCC6, CotA, CotA laccase, CotA-laccase, CotA-type laccase, CueO, DA2_0547, DcLac1, DcLac2, Diphenol oxidase, EpoA, Ery4, Ery4 laccase, FpLcc1, FpLcc2, GMET_RS10855, Hvo_B0205, LAC, Lac I, Lac II, Lac-3.5, Lac-4.8, LAC1, Lac2, Lac2a, LAC3, Lac4, LacA, Lacc, laccase, laccase 1, laccase 3, laccase A, Laccase allele OR, Laccase allele TS, laccase CueO, laccase POXA3b, laccase-2, laccase2, LacCh, lacTT, LacTv, LacZ1, Lcc, lcc1, Lcc2, Lcc3, Lcc4, Lcc9, LccA, lccdelta, lccgamma, Ligninolytic phenoloxidase, MAL, McoP, MmPPO laccase, MmPPOA, More, MSK laccase, multicopper oxidase, p-benzenediol:dioxygen oxidoreductase, p-diphenol dioxygen oxidoreductase, p-diphenol oxidase, p-diphenol: dioxygen oxidoreductase, p-diphenol:dioxygenoxidoreductase, p-diphenol:O2 oxidoreductase, p-diphenol:oxygen oxidoreductase, p-diphenol:oxygen-oxidoreductase, PCL, phenol oxidase, PM1 laccase, polyphenol oxidase A, POXA1b, POXA1w, POXA2, POXA3 laccase, POXA3a, POXA3b, POXC, PpoA, PsLac1, PsLac2, rlac1338, SLAC, SN4LAC, spore coat A protein, spore coat protein A, SRL1, SvLAC13, SvLAC15, SvLAC50, SvLAC52, SvLAC9, TaLac1, ThL, TTC1370, TthLAC, two-domain laccase, urishiol oxidase, urushiol oxidase, Wlac, YacK, yellow laccase
ECTree
1.10.3.2 laccaseAdvanced search results
results (
results (pH Stability
pH Stability on EC 1.10.3.2 - laccase
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pH STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
10
11
2 - 10
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purified enzyme, 90 min, stable at pH 6.5-9.5, 20% remaining activity at pH 2.0, 65% remaining activity at pH 4.0 and pH 10.0
701029
2 - 7
6 h, 25°C, in the absence of the substrate, recombinant laccase is stable
741665
2.5
2.5 - 10
recombinant enzyme, rLac1 retains more than 80% of its activity in solutions within this range after a 24 h incubation at 25°C
726411
3 - 10
stability of recombinant wild-type and mutant enzymes, overview
697668
3 - 11
Sporothrix carnis
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2 h, enzyme retains over 60% of its initial activity
742691
3 - 5.6
after treatment with citrate-phosphate buffer (pH 3.0-5.6) for 24 h, enzyme activity is not lost
743888
3 - 9
3.5
24 h, complete loss of activity, recombinant enzyme
742954
4 - 10
4 - 11
Thermochaetoides thermophila
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396347
4 - 6
4 - 8
4 - 9
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the purified laccase is more stable at alkaline pH of 7.0-9.0 after 24 h than at its optimum pH of 4.0
726534
4.5
half live of mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence: 63.0 min (at pH 6.0), 6.1 min (pH 10.5)
742942
4.5 - 10
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the purified enzyme is highly stable over a broad range of pH values ranging from pH 4.5 to pH 10.0, maintaining 75% of its original activity after incubation at 25°C for 72 h, it is highly stable and resistant under alkaline conditions, with pH values ranging from pH 7.0 to pH 10.0
724573
5 - 10
5 - 6
5.5
5.5 - 8
the L559A mutant remains stable within this pH range after 330 h at 4°C, the enzyme looses 60% at pH 5.0, and 95% at pH 4.0 after 330 h. No activity remains at pH 3.0 and pH 2.0 after 330 h
697913
6 - 10
6 - 7
6 - 9
6.5
7 - 11
-
the enzyme retains over 50% residual activity after 5-day incubation
741648
7.6
8.5
additional information
10
-
isozyme Lacc II is stable, isozyme Lacc I loses 10% of activity within 1 h
671514
10
-
highest stability is observed at pH 10.0 with an approximately residual activity of 90% after 5 days
741648
11
Sporothrix carnis
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2 h, no loss of activity
742691
2
PM1 laccase displays high stability at extreme pH values. It retains 60% of the initial activity after 24 h at pH 2
764876
2
activity decreases to approximately 1% at the end of 105 h incubation. t1/2: 18 h
743648
2
20°C, 2 days, the enzyme retains about 30% residual activity
742013
3
unstable at, 23 and 15% of the original activity remains after incubation for 1 and 3 days respectively
741634
3
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10 days, 75-80% loss of activity (depending on purification method)
743713
3
activity decreases to approximately 20% at the end of 105 h incubation. t1/2: 43 h
743648
3
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partial inactivation of both isozymes, 60-64% remaining activity after 1 h
671514
3
20°C, 2 days, the enzyme retains about 42% residual activity
742013
3 - 9
stable up to when using the substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
764877
3 - 9
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after 24 h incubation at pH 4.8 and 4°C, 80% of the initial activity remains. After 1 h incubation, the enzyme shows about 65% activity at pH 3.0, about 75% activity at pH 4.0, about 80% activity at pH 5.0, about 60% activity at pH 6.0, about 10% activity at pH 7.0, about 5% activity at pH 8.0, and no activity at pH 9.0
711502
4
20°C, 2 days, the enzyme retains about 65-60% residual activity
742013
4
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4 h, solgel immobilized laccase retains 98% of the activity. In comparison to soluble laccase better stability is observed in the order, solgel matrix > calcium alginatechitosan > copper alginate > calcium alginate
743017
4 - 10
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the laccase has a broad stable pH range. After 72 h incubation at 30°C, the enzyme shows a different stability pattern: the laccase activity decreases rapidly at pH 2.0, 43% remaining activity at pH 4.0 and pH 8.0, over 70% remaining activity at pH 10.0 with a half-life of more than 130 h, the laccase is more stable in an alkaline condition
695714
4 - 6
Myrioconium sp.
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15°C, 1 h, more than 80% of the initial laccase activity is retained
684626
4 - 8
the laccase is most stable at pH 4.0, pH 7.0, and 8.0, retaining about 57%, 62%, and 65% of the initial activity, respectively, after 355 h. The enzyme is less stable at pH 5.0 and pH 6.0 and is completely inactivated within 355 h at pH 2.0
695839
4 - 8
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the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4
673378
5
20°C, 2 days, the enzyme retains about 65-60% residual activity
742013
5 - 10
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stable at pH 5.0-10.0. Activity is drastically reduced at pH values lower than 5.0 after 6 h incubation
742104
5 - 10
Thermochaetoides thermophila
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1 h, stable
737487
5 - 6
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4 h, solgel immobilized laccase retains 100% of the activity. In comparison to soluble laccase better stability is observed in the order, solgel matrix > calcium alginatechitosan > copper alginate > calcium alginate
743017
6 - 10
20°C, 2 days, the enzyme retains about 70% residual activity
742013
6 - 7
-
purified enzyme, the enzyme retains 80% of its initial activity after 96 h
726416
6 - 9
-
the enzyme retains more than 80% of its activity at within 24 h at 37°C
743704
6 - 9
Phlebia fascicularia
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90% residual activity after 24 h incungation
657149
6.5
72 h, 22°C, enzyme retains 21% of its activity, recombinant enzyme
742954
6.5
wild-type enzyme Lcc9 and recombinant enzyme expressed in Pichia pastoris retain about 60% of their original activities
765709
7
-
10 days, 30-40% loss of activity (depending on purification method)
743713
7
-
4 h, solgel immobilized laccase retains 96% of the activity. In comparison to soluble laccase better stability is observed in the order, solgel matrix > calcium alginatechitosan > copper alginate > calcium alginate
743017
7.6
half live of mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence: 57.8 min (at pH 6.0)
742942
8
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10 days, 10-20% loss of activity (depending on purification method)
743713
8
24 h, residual activity of laccase 3 is 89%, residual activity of deglycosylated laccase 3 is 77%
742679
8
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4 h, solgel immobilized laccase retains 92% of the activity. In comparison to soluble laccase better stability is observed in the order, solgel matrix > calcium alginatechitosan > copper alginate > calcium alginate
743017
8.5
72 h, 22°C, enzyme retains 17% of its activity, recombinant enzyme
742954
8.5
1 h, 60°C, wild-type enzyme Lcc9 retains 11.8% of its original activity while the recombinant enzyme expressed in Pichia pastoris retains 63.2% of its original activity
765709
9
PM1 laccase displays high stability at extreme pH values. It retains 100% of the initial activity after 24 h at pH 9
764876
