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1.10.3.2: laccase

This is an abbreviated version!
For detailed information about laccase, go to the full flat file.

Word Map on EC 1.10.3.2

Reaction

4 benzenediol +

O2
= 4 benzosemiquinone + 2 H2O

Synonyms

ATM, benzenediol oxygen oxidoreductase, benzenediol-oxygen oxidoreductase, benzenediol: oxygen oxidoreductase, benzenediol:O2 oxidoreductase, Benzenediol:oxygen oxidoreductase, blue laccase, blue multicopper oxidase, CcLCC6, CotA, CotA laccase, CotA-laccase, CotA-type laccase, CueO, DA2_0547, DcLac1, DcLac2, Diphenol oxidase, EpoA, Ery4, Ery4 laccase, FpLcc1, FpLcc2, GMET_RS10855, Hvo_B0205, LAC, Lac I, Lac II, Lac-3.5, Lac-4.8, LAC1, Lac2, Lac2a, LAC3, Lac4, LacA, Lacc, laccase, laccase 1, laccase 3, laccase A, Laccase allele OR, Laccase allele TS, laccase CueO, laccase POXA3b, laccase-2, laccase2, LacCh, lacTT, LacTv, LacZ1, Lcc, lcc1, Lcc2, Lcc3, Lcc4, Lcc9, LccA, lccdelta, lccgamma, Ligninolytic phenoloxidase, MAL, McoP, MmPPO laccase, MmPPOA, More, MSK laccase, multicopper oxidase, p-benzenediol:dioxygen oxidoreductase, p-diphenol dioxygen oxidoreductase, p-diphenol oxidase, p-diphenol: dioxygen oxidoreductase, p-diphenol:dioxygenoxidoreductase, p-diphenol:O2 oxidoreductase, p-diphenol:oxygen oxidoreductase, p-diphenol:oxygen-oxidoreductase, PCL, phenol oxidase, PM1 laccase, polyphenol oxidase A, POXA1b, POXA1w, POXA2, POXA3 laccase, POXA3a, POXA3b, POXC, PpoA, PsLac1, PsLac2, rlac1338, SLAC, SN4LAC, spore coat A protein, spore coat protein A, SRL1, SvLAC13, SvLAC15, SvLAC50, SvLAC52, SvLAC9, TaLac1, ThL, TTC1370, TthLAC, two-domain laccase, urishiol oxidase, urushiol oxidase, Wlac, YacK, yellow laccase

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.2 laccase

Temperature Range

Temperature Range on EC 1.10.3.2 - laccase

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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 100
-
a cold adaptation enzyme with a relative catalytic activity of 30.2% at 0°C, but also a high thermostable enzyme. About 50-70% of relative activity at 10-30°C
10 - 35
-
-
20 - 100
-
24% of maximal activity at 100°C, 65% at 20°C, activity profile, overview
20 - 50
20 - 60
20 - 70
-
20°C: about 60% of maximal activity, 70°C: about 50% of maximal activity
20 - 75
-
activity range
20 - 80
21 - 60
21°C: 75% of maximal activity, 60°C: 40-50% of maximal activity
25 - 40
-
sharp decrease in activity above 40°C
25 - 55
-
temperature-activity profile at pH 3.8
25 - 60
25 - 65
the enzyme displays more than 50% of maximal activity between 25°C and 65°C against guaiacol, catechol or 2,6-dimethoxyphenol. At 65°C for 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), the relative activity of LacA is 45%
25 - 70
25 - 75
-
activity range
25 - 80
25 - 90
-
-
25 - 95
-
25°C: about 50% of maximal activity, 95°C: about 40% of maximal activity
30 - 100
30 - 40
-
30°C: about 50% of maximal activity, 40°C: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30 - 50
-
activity range, rapid loss at 60°C
30 - 60
-
30°C: about 55% of maximal activity, 65°C: about 55% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30 - 70
30 - 75
activity range
30 - 80
30 - 90
35 - 60
35 - 65
37 - 100
-
37°C: about 55% of maximal activity, 100°C: about 60% of maximal activity, substrate: 2,6-dimethoxyphenol
40 - 70
40 - 75
maximum activity at 50°C, retaining more than 80% of activity at temperatures between 60°C and 75°C, but less than 55% of activity at temperatures below 40°C
40 - 80
40 - 85
40°C: about 40% of maximal activity, 85°C: about 55% of maximal activity
40 - 90
-
40°C: about 60% of maximalm activity, 90°C: about 75% of maximal activity
45 - 60
45 - 75
45°C: about 45% of maximal activity, 75°C: about 55% of maximal activity, substrate: syringaldazine
45 - 80
-
45°C: about 60% of maximal activity, 80°C: about 65% of maximal activity, substrate: 2,6-dimethoxyphenol
5 - 70
-
the enzyme activity increases by about 70% to its maximum at 30°C when the temperature is elevated from 5°C, and then falls to about 10% of the maximal value when the temperature is raised to 70°C
5 - 75
-
high activity at 5°C, 25 and 30% of maximum activity at 60°C for isozymes Lacc I and II, 20% at 75°C
50 - 70
-
all the forms of immobilized laccase show more than 90% relative activity at higher temperature of 55°C and 60°C. Sol-gel immobilized laccase shows 97% (65°C) and 94% (70°C) relative activity at higher temperatures. Soluble enzyme loses maximum activity above 55°C
50 - 80
50 - 90
-
rapid increase in laccase activity from 50°C to 80°C followed by a decline inlaccase activity of approximately 26% at 90°C
55 - 90
-
55°C: about 50% of maximal activity, 90°C: about 45% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
60 - 95
-
the recombinant LacG enzyme shows more than 80% activity within 60-95°C
65 - 100
-
65°C: about 50% of maximal activity, 100°C: about 50% of maximal activity
65 - 85
FJ560721
activity range
additional information