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100000
x * 70000, recombinant nonglycosylated enzyme expressed in Trichodrma reesei, SDS-PAGE, x * 100000, recombinant glycosylated enzyme expressed in Saccharomyces cerevisiae, SDS-PAGE
107000
-
recombinant mutant WlacD
150000 - 151200
about, gel filtration and mass spectrometry
177000
-
enzyme conjugated with 2 kDa PEG, gel filtration
22400
-
2 * 22400, SDS-PAGE
269000
-
enzyme conjugated with 5 kDa PEG, gel filtration
29800
-
calculated from sequence
37000
-
2 * 37000, SDS-PAGE
38000
-
3 * 38000, SDS-PAGE after boiling for 20 min, 114000, stable migration in regular SDS-PAGE
40000
-
x * 40000, SDS-PAGE
43000
-
2 * 43000, SDS-PAGE
47000
-
1 * 47000, SDS-PAGE
48986
calculated from sequence
52000 - 59000
-
gel filtration
54100
x + 63700, glycosylated recombinant enzyme, SDS-PAGE, x * 54100, about, sequence calculation
56800
x * 56800, SDS-PAGE
57500
x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE
61400
-
x * 61400, SDS-PAGE
62700
-
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE
63397
x * 63397, calculation from sequence
63400
estimated from SDS-PAGE
64100
x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE
64200
-
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE
65000 - 68000
-
gel filtration, 3 isoenzymes
68500
-
1 * 68500, SDS-PAGE
70000 - 75000
-
gel filtration
70780
-
x * 70780, mass spectrometry, x * 69000, SDS-PAGE
71450
-
1 * 74000, SDS-PAGE, 1 * 71450, mass spectrometry
72400
-
x * 72400, SDS-PAGE, probably anomalous migration on SDS-gels
72700
Myrioconium sp.
-
matrix-assisted laser desorption/ionization-time of flightmass spectrometry
73500
-
ultracentrifugation, amino acid composition
79600
-
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE
84280
-
2 * 84280, mature enzyme, calculated from amino acid sequence
88000 - 90000
gel filtration
96000
-
1 * 96000, SDS-PAGE
110000
-
recombinant wild-type enzyme and mutant WlacS
110000
-
x * 90000, SDS-PAGE, lac90, x * 110000, SDS-PAGE, lac110
130000
-
-
130000
-
gel filtration, laccase 4
165000
-
-
175000
-
-
34000
-
SDS-PAGE
34000
-
2 * 34000, SDS-PAGE
35500 - 38000
-
gallic acid induced isozyme, sedimentation and diffusion data
35500 - 38000
-
laccase I and II, ultracentrifugation, enzymes differ in amino acid composition
36000
Thermochaetoides thermophila
-
x * 36000, SDS-PAGE
36000
Thermochaetoides thermophila
-
3 * 36000, SDs-PAGE
383000 - 390000
-
sedimentation and diffusion constants, sedimentation equilibrium centrifugation
383000 - 390000
-
laccase I, disc gel electrophoresis, interconversion of laccases I, III, IV
43700
-
-
43700
-
x * 43700, SDS-PAGE
45000
-
2 * 45000, SDS-PAGE
46000
-
gel filtration
46000
-
2 * 46000, SDS-PAGE
52000
-
laccase 1, gel filtration
52000
-
free enzyme, gel filtration
53000
-
gel filtration
53000
SDS-PAGE, recombinant enzyme
53000
-
x * 53000, SDS-PAGE
53000
x * 58000, glycosylated enzyme, SDS-PAGE, x * 53000, deglycosylated enzyme, SDS-PAGE
54000
-
gel filtration
54000
Coriolisimus fulvocinerea
-
gel filtration
55000
-
gel filtration
55000
-
laccase 2, gel filtration
55000
-
gel filtration, laccase I and II
55000
-
1 * 55000, SDS-PAGE, isoenzymes I and II
55000
x * 55000, native enzyme, SDS-PAGE
57000
-
gel filtration
57000
-
1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE
57000
-
x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE
58000
-
gel filtration
58000
calculated from sequence, SDS-PAGE
58000
-
1 * 58000, SDS-PAGE
58000
-
x * 58000, SDS-PAGE
58000
x * 58000, glycosylated enzyme, SDS-PAGE, x * 53000, deglycosylated enzyme, SDS-PAGE
58000
-
x * 58000, recombinant CotA-type laccase, SDS-PAGE
58500
-
58500
1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry
59000
-
gel filtration
59000
-
indicated by gel filtration chromatography
59000
-
x * 59000, SDS-PAGE
59000
-
x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE
59000
-
1 * 59000, isoform POXC, SDS-PAGE
60000
-
gel filtration
60000
Phlebia fascicularia
-
gel filtration, isozymes Lac I and Lac II
60000
-
x * 60000, SDS-PAGE
61000
-
gel filtration and native PAGE
61000
-
x * 61000, SDS-PAGE
61000
-
1 * 61000, isoform POXA1w, SDS-PAGE
62000
-
gel filtration
62000
calculated from sequence
62000
-
x * 62000, SDS-PAGE
62000
1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry
62000
-
1 * 62000, isoform POXA1b, SDS-PAGE
63000
-
gel filtation
63000
calculated from sequence
63000
-
1 * 63000, SDS-PAGE
63000
-
x * 63000, SDS-PAGE
63000
-
x * 63000, SDS-PAGE, main isoform
63000
-
2 * 63000, recombinant enzyme, SDS-PAGE
63000
-
x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE
64000
-
gel filtration
64000
-
1 * 64000, SDS-PAGE
64000
-
1 * 64000, SDS-PAGE
64000
-
x * 64000, SDS-PAGE
64000
-
1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE
64000 - 65000
-
gel filtration
64000 - 65000
-
sedimentation equilibrium centrifugation
64800
Loweporus lividus MTCC-1178
-
-
64800
Loweporus lividus
-
calculated from amino acid sequence
65000
-
-
65000
-
native enzyme, gel filtration
65000
Loweporus lividus
-
SDS-PAGE
65000
-
SDS-PAGE, gel filtration
65000
-
x * 65000, SDS-PAGE
65000
-
x * 65000, SDS-PAGE
65000
MALDI/TOF-TOF analyses, PM1 laccase
66000
Bacillus spp.
-
-
66000
-
non-denaturing PAGE
66000
-
x * 66000, SDS-PAGE
66000
-
1 * 66000, SDS-PAGE
66000
-
2 * 66000, SDS-PAGE, laccase 4
67000
-
-
67000
-
x * 67000 + x * 16000-18000, SDS-PAGE and MALDI-TOF, both POXA3a and POXA3b
67000
Fomes sclerodermeus
-
x * 67000, both isozymes, SDS-PAGE
67000
-
1 * 67000, isoform POXA2, SDS-PAGE
68000
-
gel filtration
68000
-
native enzyme, gel filtration
68000
-
1 * 68000, SDS-PAGE
68000
x * 68000, SDS-PAGE
69000
-
1 * 69000, SDS-PAGE
69000
-
1 * 69000, SDS-PAGE
69000
-
x * 70780, mass spectrometry, x * 69000, SDS-PAGE
70000
-
70000
-
laccase 3, gel filtration
70000
-
2 * 70000, SDS-PAGE
70000
-
x * 70000, SDS-PAGE
70000
-
x * 70000, SDS-PAGE
70000
x * 70000, SDS-PAGE
70000
-
1 * 70000, SDS-PAGE, isoenzyme L2
70000
x * 70000, recombinant nonglycosylated enzyme expressed in Trichodrma reesei, SDS-PAGE, x * 100000, recombinant glycosylated enzyme expressed in Saccharomyces cerevisiae, SDS-PAGE
70000
x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE
71000
-
gel filtration
71000
-
native PAGE and gel filtration
71000
-
1 * 71000, SDS-PAGE
71000
-
x * 71000, enzyme from strain KUM 60953, SDS-PAGE, x * 74000, enzyme from strain KUM 60954, SDS-PAGE
72000
-
-
73000
-
gel filtration, isoenzyme L2
73000
-
1 * 73000, SDS-PAGE
73000
-
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE
74000
-
-
74000
-
1 * 74000, SDS-PAGE, 1 * 71450, mass spectrometry
74000
-
x * 71000, enzyme from strain KUM 60953, SDS-PAGE, x * 74000, enzyme from strain KUM 60954, SDS-PAGE
75000
-
-
75000
-
nondenaturing PAGE
76000
-
2 * 76000, SDS-PAGE
76000
-
2 * 76000, SDS-PAGE, N-terminal amino acid sequence
77000
Thermochaetoides thermophila
-
-
77000
Thermochaetoides thermophila
-
x * 77000, SDS-PAGE
77000
-
1 * 77000, isoenzyme L1, SDS-PAGE
79000
-
x * 79000, SDS-PAGE
79000
-
x * 79000, SDS-PAGE, isozyme pI 3.60
80000
-
gel filtration
80000
-
laccase IV, disc gel electrophoresis
80000
-
x * 80000, SDS-PAGE, enzyme differs from white-spore and green-spore strains
82000
FJ560721
gel filtration
82000
-
gel filtration, isoenzyme L1
85000
-
-
85000
-
enzyme conjugated with 1.1 kDa PEG, gel filtration
85000
-
1 * 85000, SDS-PAGE
90000
-
-
90000
-
x * 90000, SDS-PAGE
90000
-
x * 90000, SDS-PAGE, lac90, x * 110000, SDS-PAGE, lac110
90000
x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE
92000
-
gel filtration
96000 - 110000
-
gel filtration
96000 - 110000
-
gel filtration
96000 - 110000
Thermochaetoides thermophila
-
gel filtration
96000 - 110000
-
sucrose density gradient centrifugation, gel filtration
96000 - 110000
-
sedimentation velocity, calculation from copper content, amino acid composition
96000 - 110000
-
sedimentation and diffusion constants
96000 - 110000
-
sedimentation and diffusion constants
96000 - 110000
-
gel filtration, electrophoresis results in 72400 Da
97000
-
97000
-
2 * 97000, SDS-PAGE
97000
-
1 * 97000, SDS-PAGE
additional information
-
overview
additional information
-
overview