1.1.99.35: soluble quinoprotein glucose dehydrogenase

This is an abbreviated version!
For detailed information about soluble quinoprotein glucose dehydrogenase, go to the full flat file.

Word Map on EC 1.1.99.35

1.1.99.35

calcoaceticus

acinetobacter

aldose

pqqh2

electrodes

pqq-containing

tautomerization

hydride

pqq-dependent

synthesis

stopped-flow

beta-anomer

biofuel production

half-reaction

gluconolactone

disaccharide

electrochemical

beta-strands

energy production

diagnostics

biotechnology

Reaction

Synonyms

2,7,9-tricarboxypyrroloquinoline quinone-dependent glucose dehydrogenase, GCD, GDH, GDHB, mGDH, PQQ dependent soluble glucose dehydrogenase, PQQ glucose dehydrogenase, PQQ-dependent glucose dehydrogenase, PQQ-GDH, PQQ-glucose dehydrogenase, PQQ-sGDH, PQQGDH, PQQGDH-B, pyrroloquinoline quinone soluble glucose dehydrogenase, pyrroloquinoline quinone-dependent glucose dehydrogenase, pyrroloquinolinequinone-dependent glucose dehydrogenase, s-GDH, sGDH, sGDH-PQQ, soluble PQQ-dependent glucose dehydrogenase, soluble PQQ-GDH, soluble PQQ-glucose dehydrogenase, soluble pyrroloquinoline quinone-dependent glucose dehydrogenase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.99 With unknown physiological acceptors

1.1.99.35 soluble quinoprotein glucose dehydrogenase

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Results	in table
22	AA Sequence
7	Application
7	Cloned(Commentary)
15	Cofactor
4	Crystallization (Commentary)
3	Expression
4	General Information
1	General Stability
4	Inhibitors
65	kcat/KM [mM/s]
3	Ki Value [mM]
67	KM Value [mM]
7	Localization
13	Metals/Ions
2	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
73	Organism
2	pH Optimum
1	Posttranslational Modification
31	Protein Variants
1	Purification (Commentary)
2	Reaction
32	Reference
2	Source Tissue
4	Specific Activity [micromol/min/mg]
94	Substrates and Products (Substrate)
5	Subunits
38	Synonyms
1	Systematic Name
7	Temperature Stability [°C]
40	Turnover Number [1/s]

KM Value

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KM Value on EC 1.1.99.35 - soluble quinoprotein glucose dehydrogenase

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88 - 90

2-deoxy-D-glucose

2 entries

2 - 46

3-O-methyl-D-glucose

7 entries

21 - 38.7

D-allose

7 entries

2.7 - 9

D-galactose

7 entries

0.74 - 64

D-glucose

18 entries

14.3 - 34

D-lactose

5 entries

14.3 - 46.5

D-maltose

5 entries

D-mannose

2 entries

7.5 - 14.3

D-xylose

2 entries

0.0014 - 20

lactose

8 entries

13 - 14

maltose

2 entries

additional information

Acinetobacter calcoaceticus

under conditions that avoid its masking by sugar-substrate inhibition as much as possible, negative kinetic cooperativity with respect to sugar substrate oxidation is observed. The cooperativity effect dramatically changes the performance of soluble GDH, as reflected by the V2 and K2 values for glucose in phosphate buffer being about 10-fold and 100-fold higher than the V1 and K1 values, respectively. Substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose

703532