1.1.99.31: (S)-mandelate dehydrogenase
This is an abbreviated version!
For detailed information about (S)-mandelate dehydrogenase, go to the full flat file.
Word Map on EC 1.1.99.31
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1.1.99.31
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s-mandelate
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putida
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benzoylformate
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fmn-dependent
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benzaldehyde
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calcoaceticus
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fmn
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mitra
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alpha-hydroxy
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l-lactate
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phenylglyoxylate
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carbanion
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flavocytochrome
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membrane-binding
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flavoenzyme
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alpha-protons
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half-reaction
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rhodotorula
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graminis
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electron-rich
- 1.1.99.31
- s-mandelate
- putida
- benzoylformate
-
fmn-dependent
- benzaldehyde
- calcoaceticus
- fmn
- mitra
-
alpha-hydroxy
- l-lactate
- phenylglyoxylate
-
carbanion
-
flavocytochrome
-
membrane-binding
-
flavoenzyme
-
alpha-protons
-
half-reaction
-
rhodotorula
- graminis
-
electron-rich
Reaction
Synonyms
(S)-mandelate dehydrogenase, L-mandelate dehydrogenase, L-MDH, MDH, SManDH, SMDH
ECTree
1.1.99.31 (S)-mandelate dehydrogenaseAdvanced search results
results (
results (KM Value
KM Value on EC 1.1.99.31 - (S)-mandelate dehydrogenase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
22
(R,S)-2-hydroxy-3-butynoic acid
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
32
(R,S)-2-hydroxybutyrate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
4.9
(R,S)-2-hydroxyhexanoate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
4.3
(R,S)-2-hydroxyisocaproate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
9.5
(R,S)-2-hydroxyisovalerate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.75
(R,S)-2-hydroxyoctanoate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
15.3
(R,S)-2-hydroxyvalerate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.9
(R,S)-3-indolelactate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.4
(R,S)-indoleglycolate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.33
(R,S)-mandelate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
10.3
2-Hydroxy-3-butenoate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
2.6
2-Hydroxyisovalerate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
2
DL-3-phenyllactate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.04
(S)-Mandelate
-
pH 7.5, 20°C, mutant enzyme, G81A/MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach and a G81A mutation in MDH
0.085
(S)-Mandelate
-
pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.09
(S)-Mandelate
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pH 7.5, 20°C, mutant enzyme MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.12
(S)-Mandelate
wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5
0.158
(S)-Mandelate
-
pH 7.5, (S)-mandelate dehydrogenase with 17 residues deleted at the carboxy terminus
0.18
(S)-Mandelate
-
pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, wild-type enzyme
0.18
(S)-Mandelate
-
pH 7.5, 25°C, electron acceptor: 2,6-dichloroindophenol
0.225
(S)-Mandelate
-
pH 7.5, (S)-mandelate dehydrogenase with residues 2-4 deleted
0.229
(S)-Mandelate
-
pH 7.5, chimeric mutant of (S)-mandelate dehydrogenase with membrane anchoring loop replaced by a portion of glycolate oxidase from spinach
5.6
(S)-Mandelate
mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5
17
(S)-Mandelate
mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5
0.78
(S)-phenyllactate
-
pH 7.5, chimeric mutant of (S)-mandelate dehydrogenase with membrane anchoring loop replaced by a portion of glycolate oxidase from spinach
17.2
2-Hydroxy-3-butynoate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
13.2
2-Hydroxybutyrate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.89
2-hydroxyhexanoate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.49
2-hydroxyisocaproate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.29
2-Hydroxyoctanoate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
3.2
2-hydroxyvalerate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.64
3-indolelactate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
1.3
3-Phenyllactate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.47
DL-[2-1H]-mandelate
-
pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme
0.74
DL-[2-1H]-mandelate
-
pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain
0.78
DL-[2-1H]-mandelate
-
pH 7.5, 25°C, cosubstrate cytochrome c, LMDH holoenzyme
0.53
DL-[2-2H]-mandelate
-
pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme
0.79
DL-[2-2H]-mandelate
-
pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain
1.33
DL-[2-2H]-mandelate
-
pH 7.5, 25°C, cosubstrate cytochrome, LMDH holoenzyme
2.7
ethyl-(S)-mandelate
mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5
2.9
ethyl-(S)-mandelate
wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5
3.7
ethyl-(S)-mandelate
mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5
0.24
indoleglycolate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.14
Mandelate
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pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
7.4
methyl-(S)-mandelate
mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5
10.3
methyl-(S)-mandelate
mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5
11.3
methyl-(S)-mandelate
wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5
0.49
O2
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pH 7.5, 20°C, mutant enzyme, G81A/MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach and a G81A mutation in MDH
0.68
O2
-
pH 7.5, 20°C, mutant enzyme MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with 205 residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
