1.1.99.28: glucose-fructose oxidoreductase
This is an abbreviated version!
For detailed information about glucose-fructose oxidoreductase, go to the full flat file.
Word Map on EC 1.1.99.28
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1.1.99.28
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zymomonas
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mobilis
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sorbitol
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gluconic
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lactobionic
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nadp-containing
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gluconolactonase
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d-xylose
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1,5-anhydro-d-fructose
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synthesis
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dihydrodiol
- 1.1.99.28
- zymomonas
- mobilis
- sorbitol
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gluconic
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lactobionic
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nadp-containing
- gluconolactonase
- d-xylose
- 1,5-anhydro-d-fructose
- synthesis
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dihydrodiol
Reaction
Synonyms
EC 1.1.1.99, GFOD2, GFOR, glucose fructose oxidoreductase, glucose-fructose oxidoreductase, glucose-fructose oxidoreductase domain containing 2, Glucose-fructose transhydrogenase, NADP(H)-dependent glucose-fructose oxidoreductase, Transhydrogenase, glucose-fructose
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Natural Substrates Products
Natural Substrates Products on EC 1.1.99.28 - glucose-fructose oxidoreductase
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REACTION DIAGRAM
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enzyme is responsible for sorbitol production
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D-Glucose + D-fructose
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the enzyme produces the solute sorbitol by reduction of fructose, coupled with the oxidation of glucose to gluconolactone and thereby protects the bacterium against osmotic shock in sugar-rich environment
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D-glucono-1,5-lactone + D-sorbitol
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D-glucose + D-fructose
D-glucono-1,5-lactone + D-sorbitol
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a variable pH from 7.8 to 6.4 and a constant temperature of about 47°C are the best conditions for achieving good conversion yields and productivities
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D-glucose + D-fructose
D-glucono-1,5-lactone + D-sorbitol
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at pH 6.4 and 39°C when 700 mM lactose/350 mM fructose pair substrate is used, the maximum possible conversion yield is attained after 24 h of operation
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D-glucose + D-fructose
D-glucono-1,5-lactone + D-sorbitol
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from pineapple juice, at optimal conditions a maximum of 80% (w/v) sugar conversion is obtained
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D-glucose + D-fructose
D-glucono-1,5-lactone + D-sorbitol
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a variable pH from 7.8 to 6.4 and a constant temperature of about 47°C are the best conditions for achieving good conversion yields and productivities
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D-glucose + D-fructose
D-glucono-1,5-lactone + D-sorbitol
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at pH 6.4 and 39°C when 700 mM lactose/350 mM fructose pair substrate is used, the maximum possible conversion yield is attained after 24 h of operation
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D-glucose + D-fructose
D-glucono-1,5-lactone + D-sorbitol
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D-glucose + D-fructose
D-glucono-1,5-lactone + D-sorbitol
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D-glucose + D-fructose
D-gluconolactone + D-glucitol
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D-glucose + D-fructose
D-gluconolactone + D-glucitol
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D-glucose + D-fructose
D-gluconolactone + D-glucitol
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D-glucono-1,5-lactone + D-sorbitol
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pineapple juice sugars, the enzyme is efficient in converting juice sugars of fruits juices having higher natural pH
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D-glucose + D-fructose + H2O
D-glucono-1,5-lactone + D-sorbitol
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pineapple juice sugars, the enzyme is efficient in converting juice sugars of fruits juices having higher natural pH
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in periplasm the enzyme functions to produce sorbitol for osmoprotection
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additional information
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decreasing enzyme/substrate affnities are observed when D-fructose is in the mixture with D-glucose, D-maltose, D-galactose, and lactose
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additional information
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decreasing enzyme/substrate affnities are observed when D-fructose is in the mixture with D-glucose, D-maltose, D-galactose, and lactose
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