1.1.99.21: D-sorbitol dehydrogenase (acceptor)
This is an abbreviated version!
For detailed information about D-sorbitol dehydrogenase (acceptor), go to the full flat file.
Word Map on EC 1.1.99.21
Reaction
Synonyms
D-sorbitol dehydrogenase, FAD-SLDH, GoSLDH, mSLDH, PQQ-dependent D-sorbitol dehydrogenase, SDH, SLDH, sldhAB, sorbitol dehydrogenase
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1.1.99.21 D-sorbitol dehydrogenase (acceptor)Advanced search results
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results (Substrates Products
Substrates Products on EC 1.1.99.21 - D-sorbitol dehydrogenase (acceptor)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-(2-hydroxyethyl)amino-1-deoxy-D-sorbitol + acceptor
6-(2-hydroxyethyl)amino-6-deoxy-L-sorbose + reduced acceptor
1-benzyloxycarbonylamino-1-deoxy-D-sorbitol + acceptor
N-benzyloxycarbonyl-6-amino-L-sorbose + reduced acceptor
-
-
-
-
?
D-mannitol + acceptor
? + reduced acceptor
-
oxidation at 5% the rate of D-sorbitol
-
-
?
D-sorbitol + phenazine methosulfate
?
-
the catalytic reaction follows an ordered Bi Bi mechanism, the native mSLDH bears two different substrate-binding sites, one for ubiquinone using as electron acceptor and the other for D-sorbitol, in addition to PQQ-binding and Mg2+-binding sites in the catalytic center
-
-
?
1-(2-hydroxyethyl)amino-1-deoxy-D-sorbitol + acceptor
6-(2-hydroxyethyl)amino-6-deoxy-L-sorbose + reduced acceptor
-
-
-
-
?
1-(2-hydroxyethyl)amino-1-deoxy-D-sorbitol + acceptor
6-(2-hydroxyethyl)amino-6-deoxy-L-sorbose + reduced acceptor
-
-
-
-
?
D-sorbitol + acceptor
L-sorbose + reduced acceptor
-
production of L-sorbose is similar in the wild-type strain and the FAD-SLDH defective strain, the enzyme is induced by L-sorbose and by D-sorbitol, FAD-SLDH links preferably to the cyanide-insensitive terminal oxidase
-
-
?
D-sorbitol + acceptor
L-sorbose + reduced acceptor
-
production of L-sorbose is similar in the wild-type strain and the FAD-SLDH defective strain, the enzyme is induced by L-sorbose and by D-sorbitol, FAD-SLDH links preferably to the cyanide-insensitive terminal oxidase
-
-
?
D-sorbitol + acceptor
L-sorbose + reduced acceptor
-
-
-
?
D-sorbitol + acceptor
L-sorbose + reduced acceptor
-
high specificity
-
-
?
D-sorbitol + acceptor
L-sorbose + reduced acceptor
-
high specificity, the following dyes act in vitro as acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, potassium ferricyanide, nitro blue tetrazolium or tetramethyl-p-phenylenediamine
-
?
D-sorbitol + acceptor
L-sorbose + reduced acceptor
D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase
the oxidation product accumulates in the culture medium
-
?
D-sorbitol + acceptor
L-sorbose + reduced acceptor
-
-
-
?
D-sorbitol + acceptor
L-sorbose + reduced acceptor
D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase
the oxidation product accumulates in the culture medium
-
?
L-glucitol + NAD+
D-sorbose + NADH + H+
-
90% conversion
-
-
?
additional information
?
-
-
no oxidation of D-arabitol, L-iditol, meso-erythritol, galactitol, dulcitol, ribitol, xylitol
-
-
?
additional information
?
-
-
no oxidation of D-arabitol, L-iditol, meso-erythritol, galactitol, dulcitol, ribitol, xylitol
-
-
?
additional information
?
-
-
production of 5-keto-D-gluconate is solely dependent on enzyme
-
-
?
additional information
?
-
-
this SLDH is distinguished from other L-sorbose-producing enzymes by its high activity and substrate specificity. Isothermal titration calorimetry shows that the protein binds more strongly to D-sorbitol than other L-sorbose-producing enzymes, and substrate docking analysis confirms a higher turnover rate
-
-
?
additional information
?
-
-
binding mode of D-sorbitol with sorbitol dehydrogenase using QM-polarized ligand docking and molecular dynamics simulations, His302, Met366, and Asp368 actively participate in D-sorbitol binding, H302 directly forms hydrogen bonds with D-sorbitol and the role of His302 is to hold the D-sorbitol, overview
-
-
?
additional information
?
-
-
GoSLDH is highly specific towards D-sorbitol, mannitol, and D-arabinitol (Table S2). No activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol
-
-
?
additional information
?
-
-
this SLDH is distinguished from other L-sorbose-producing enzymes by its high activity and substrate specificity. Isothermal titration calorimetry shows that the protein binds more strongly to D-sorbitol than other L-sorbose-producing enzymes, and substrate docking analysis confirms a higher turnover rate
-
-
?
additional information
?
-
-
GoSLDH is highly specific towards D-sorbitol, mannitol, and D-arabinitol (Table S2). No activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol
-
-
?
