1.1.99.21: D-sorbitol dehydrogenase (acceptor)
This is an abbreviated version!
For detailed information about D-sorbitol dehydrogenase (acceptor), go to the full flat file.
Reaction
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=
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Synonyms
D-sorbitol dehydrogenase, FAD-SLDH, GoSLDH, mSLDH, PQQ-dependent D-sorbitol dehydrogenase, SDH, SLDH, sldhAB, sorbitol dehydrogenase
ECTree
Purification
Purification on EC 1.1.99.21 - D-sorbitol dehydrogenase (acceptor)
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by cell disruption and ultracentrifugation
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by CM-cellulose and sephacryl HR 400 column chromatography
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ion exchange chromatography, gel filtration
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metal affinity column chromatography
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recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)-CodonPlus RIL
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