1.1.99.21: D-sorbitol dehydrogenase (acceptor)

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For detailed information about D-sorbitol dehydrogenase (acceptor), go to the full flat file.

Reaction

Synonyms

D-sorbitol dehydrogenase, FAD-SLDH, GoSLDH, mSLDH, PQQ-dependent D-sorbitol dehydrogenase, SDH, SLDH, sldhAB, sorbitol dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.99 With unknown physiological acceptors

                1.1.99.21 D-sorbitol dehydrogenase (acceptor)

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Results	in table
497	AA Sequence
8	Application
1	CAS Registry Number
5	Cloned(Commentary)
16	Cofactor
1	Crystallization (Commentary)
2	Expression
7	General Information
3	General Stability
7	Inhibitors
1	kcat/KM [mM/s]
9	KM Value [mM]
10	Localization
2	Metals/Ions
11	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
30	Organism
6	Pathway
6	pH Optimum
2	pH Range
7	Protein Variants
7	Purification (Commentary)
2	Reaction
4	Reaction Type
23	Reference
5	Specific Activity [micromol/min/mg]
3	Storage Stability
41	Substrates and Products (Substrate)
10	Subunits
31	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
1	Turnover Number [1/s]

Cloned

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Cloned on EC 1.1.99.21 - D-sorbitol dehydrogenase (acceptor)

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Gluconobacter oxydans

-

695746

expressed in Escherichia coli BL21-Gold (DE3) cells

Bradyrhizobium japonicum

-

739825

gene sldh, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)-CodonPlus RIL

Gluconobacter oxydans

-

741426

gene sldhAB, enzyme overexpression in an industrial strain Gluconobacter oxydans WSH-003 with a strong promoter PtufB, subcloning in Escherichia coli strain JM109, quantitative real-time PCR expression analysis

Gluconobacter oxydans

Q70JP0; Q70JN9

740961

in Escherichia coli, plasmids harboring the disrupted gene are introduced into Gluconobacter frateurii THD32 by electroporation

Gluconobacter frateurii

-

685690