1.1.99.18: cellobiose dehydrogenase (acceptor)
This is an abbreviated version!
For detailed information about cellobiose dehydrogenase (acceptor), go to the full flat file.
Word Map on EC 1.1.99.18
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1.1.99.18
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cellulose
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phanerochaete
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chrysosporium
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biofuels
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basidiomycete
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flavocytochrome
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lignocellulose
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white-rot
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laccase
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pyranose
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corynascus
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lpmos
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myriococcum
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wood-degrading
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trametes
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cellodextrins
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thermophilum
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ligninolytic
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cello-oligosaccharides
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cellulose-binding
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glucose-methanol-choline
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cellulose-degrading
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flavodehydrogenase
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analysis
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sporotrichum
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rolfsii
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insolens
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myrothecium
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cellulose-grown
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biofuel production
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biotechnology
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industry
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dichlorophenol-indophenol
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brown-rot
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pycnoporus
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cellulose-based
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humicola
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wood-rotting
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bioanode
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verrucaria
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cellobiohydrolases
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diagnostics
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medicine
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synthesis
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degradation
- 1.1.99.18
- cellulose
- phanerochaete
- chrysosporium
-
biofuels
-
basidiomycete
-
flavocytochrome
- lignocellulose
-
white-rot
- laccase
- pyranose
- corynascus
-
lpmos
-
myriococcum
-
wood-degrading
- trametes
- cellodextrins
- thermophilum
-
ligninolytic
- cello-oligosaccharides
-
cellulose-binding
-
glucose-methanol-choline
-
cellulose-degrading
-
flavodehydrogenase
- analysis
- sporotrichum
- rolfsii
- insolens
-
myrothecium
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cellulose-grown
- biofuel production
- biotechnology
- industry
- dichlorophenol-indophenol
-
brown-rot
- pycnoporus
-
cellulose-based
- humicola
-
wood-rotting
-
bioanode
- verrucaria
- cellobiohydrolases
- diagnostics
- medicine
- synthesis
- degradation
Reaction
Synonyms
cbdA, CBO, CBOR, Cdh, CDH IIA, CDH IIB, cdh-1, CDH1, Cdh2, CDHIIA, cellobiose (acceptor) 1-oxidoreductase, cellobiose dehydrogenase, cellobiose dehydrogenase IIA, cellobiose oxidase, cellobiose oxidoreductase, cellobiose [acceptor] 1-oxidoreductase, Cellobiose-quinone oxidoreductase, cellobiose:(acceptor) 1-oxidoreductase, cellobiose:quinone oxidoreductase, cellobiose:[acceptor] 1-oxidoreductase, DCHsr, dehydrogenase, cellobiose, EC 1.1.3.25, EC 1.1.5.1, MtCDH, oxidase, cellobiose, Thite_59724, TpCDH, TvCDH
ECTree
1.1.99.18 cellobiose dehydrogenase (acceptor)Advanced search results
results (
results (Temperature Stability
Temperature Stability on EC 1.1.99.18 - cellobiose dehydrogenase (acceptor)
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
24
30
30 - 50
30 - 60
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the enzyme is highly stable at 30-60°C, with maximum activity at 30°C when incubated for 5 min. Below 30°C and above 60°C, the enzyme activity decreases, respectively
35
37
Thermothelomyces myriococcoides
residual activity after 52 h when stored in 11 mM PBS pH 7.4 buffer: wild-type enzyme (106%), mutant enzyme M309V: (103%), mutant enzyme M309K: (94%), mutant enzyme M409S: (102%), mutant enzyme M309K/M409S: (116%), mutant enzyme L324C: (57%), mutant enzyme L324C/M409S: (58%), mutant enzyme M309K/L324C: (8%)
40
40 - 60
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20% activity lost after 12 h at 50°C and pH 6.1, 80% activity lost after 1 h at 60°C and pH 6.1, all activity lost after 10 min at 70°C and pH 6.1
45
50
50 - 70
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thermostable enzyme, 10 h half-life at 60°C, 3 h at 70°C
50 - 90
complete loss of enzyme activity is recorded at 90°C after heat exposure for 30 min and at 50°C after 12 h
55
60
65 - 87
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the enzyme unfolds over the range of 65-87°C, with a Tm value of 80.2°C
70
80
30 - 50
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the enzyme is thermostable at 30°C and loses approximately 20-25% of its activity after 4 h at 40°C and half of its activity after 30 min at 50°C
30 - 50
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the enzyme is thermostable at 30°C and loses approximately 20-25% of its activity after 4 h at 40°C and half of its activity after 17 min at 50°C
35
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loss of 40% activity after 8 h at 45°C, enzyme is almost completely inactivated after 4 h at 50°
40
240 min, about 20% loss of activity of wild-type enzyme, about 30% loss of activity of recombinant enzyme
50
-
50% of activity is lost when incubated at 50°C for 30 min at pH 5.0
50
60 min, about 55% loss of activity of wild-type enzyme and recombinant enzyme
55
-
half-inactivation time of CDH at pH 6 ca. 100 min, stabilty at pH 8 and pH 4 is lower
55
20 min, about 60% loss of activity of wild-type enzyme and recombinant enzyme
60
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25 min half-life at 60°C, instantaneousley inactivated at 70°C
