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1.1.5.9: glucose 1-dehydrogenase (FAD, quinone)

This is an abbreviated version!
For detailed information about glucose 1-dehydrogenase (FAD, quinone), go to the full flat file.

Word Map on EC 1.1.5.9

Reaction

D-glucose
+
a quinone
=
D-glucono-1,5-lactone
+
a quinol

Synonyms

D-glucose:acceptor 1-oxidoreductase, dehydrogenase, glucose (acceptor), dehydrogenase, glucose (Aspergillus), EC 1.1.99.10, FAD dependent glucose dehydrogenase, FAD glucose dehydrogenase, FAD-dependent GDH, FAD-dependent glucose dehydrogenase, FAD-GDH, FAD-glucose dehydrogenase, FAD-glucose dehydrogenases, FADGDH, FADGH, flavin adenine dinucleotide dependent glucose dehydrogenase, flavin adenine dinucleotide-dependent glucose dehydrogenase, GDH, GDH-FAD, GLD, glucose dehydrogenase, glucose dehydrogenase (Aspergillus), glucose dehydrogenase (decarboxylating), thermostable GDH, thermostable glucose dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.5 With a quinone or similar compound as acceptor
                1.1.5.9 glucose 1-dehydrogenase (FAD, quinone)

Molecular Weight

Molecular Weight on EC 1.1.5.9 - glucose 1-dehydrogenase (FAD, quinone)

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
118000
-
low speed sedimentation without reaching equilibrium
120000
-
gel filtration
13000
-
SDS-PAGE, X * 60000 (alpha-subunit), x * 40000 (beta-subunit), x * 18000 (gamma-subunit), x * 13000 (gamma-subunit)
18000
350000
-
native PAGE
380000
-
gel filtration chromatography
40000
-
SDS-PAGE, X * 60000 (alpha-subunit), x * 40000 (beta-subunit), x * 18000 (gamma-subunit), x * 13000 (gamma-subunit)
43000
58000
x * 58000, SDS-PAGE
59800
-
1 * 59800 + 1 * 18000, SDS-PAGE
60000
67000
68000
-
1 * 68000, deglycosylated enzyme, SDS-PAGE
85000
-
after preincubation at 70°C for 30 min, native PAGE
86000
-
calculation from titration of the prosthetic group with glucose
87000
-
1 * 87000, monomeric in presence of 1% Triton X-100, aggregation after removing the detergent, urea-SDS-PAGE
88000 - 131000
-
-
93000
-
sucrose density gradient centrifugation, in presence of Triton X-100
95000 - 135000
97000
-
deglycosylated enzyme, gel filtration