1.1.5.8: quinate/shikimate dehydrogenase (quinone)

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For detailed information about quinate/shikimate dehydrogenase (quinone), go to the full flat file.

Reaction

Synonyms

dehydrogenase, quinate (pyrroloquinoline-quinone), EC 1.1.99.25, NAD(P)+-independent quinate dehydrogenase, NAD(P)-independent quinate dehydrogenase, PQQ-dependent dehydrogenase, PQQ-QDH, pyrroloquinoline quinone-dependent dehydrogenase, QDH, quiA, quinate dehydrogenase, quinate dehydrogenase (quinone), quinate:pyrroloquinoline-quinone 5-oxidoreductase, quinoprotein quinate dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.5 With a quinone or similar compound as acceptor

                1.1.5.8 quinate/shikimate dehydrogenase (quinone)

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Results	in table
558	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
8	Cofactor
7	General Information
2	Inhibitors
5	KM Value [mM]
10	Localization
1	Metals/Ions
2	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
32	Organism
7	Pathway
5	pH Optimum
3	Purification (Commentary)
1	Reaction
3	Reaction Type
26	Reference
9	Source Tissue
2	Specific Activity [micromol/min/mg]
43	Substrates and Products (Substrate)
11	Subunits
55	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]

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Cloned on EC 1.1.5.8 - quinate/shikimate dehydrogenase (quinone)

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

gene qdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression in pyrroloquinoline quinone synthesizing Pseudomonas putida strain HK5

Gluconobacter oxydans

B9TTF1

711180

gene quiA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, heterologous expression of QDH in Gluconobacter frateurii, improvement of the heterologous expression of QDH in Gluconobacter strains using a broad-host-range plasmid, best with a long 5'-UTR. Gluconobacter oxydans strain NBRC12528 DELTAadhA::KmR is transformed by triparental mating using Escherichia coli HB101 harboring pRK2013 as the helper strain, Gluconobacter frateurii strain SEI46 (DELTAadhAB) is transformed with the same plasmids by electroporation

Gluconobacter oxydans

A0A2Z5U248, A0A2Z5U421

762315

gene quiA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, QDH translates from the second TTG codon: Met-His-Ser-Ile-Asp, heterologous expression of QDH in Gluconobacter frateurii, improvement of the heterologous expression of QDH in Gluconobacter strains using a broad-host-range plasmid, best with a long 5'-UTR. Gluconobacter oxydans strain NBRC12528 DELTAadhA::KmR is transformed by triparental mating using Escherichia coli HB101 harboring pRK2013 as the helper strain, Gluconobacter frateurii strain SEI46 (DELTAadhAB) is transformed with the same plasmids by electroporation. Change of the initiation codon to ATG does not improve QDH activity but results in expression levels similar to those achieved with the wild-type plasmid

Gluconobacter oxydans

A0A2Z5U248, A0A2Z5U421

762315