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1.1.5.2: glucose 1-dehydrogenase (PQQ, quinone)

This is an abbreviated version!
For detailed information about glucose 1-dehydrogenase (PQQ, quinone), go to the full flat file.

Word Map on EC 1.1.5.2

Reaction

D-glucose
+
ubiquinone
=
D-glucono-1,5-lactone
 +
ubiquinol

Synonyms

(PQQ)GDH, aldose sugar dehydrogenase, Asd, beta-D-glucose:(acceptor) 1-oxidoreductase, D-glucose:(pyrroloquinoline-quinone) 1-oxidoreductase, dehydrogenase, glucose (pyrroloquinoline-quinone), EC 1.1.99.17, GCD, GDH, GDH-B, GDH1, GdhA, GDHm, GlcDH, glucose dehydrogenase, glucose dehydrogenase (PQQ dependent), glucose dehydrogenase (pyrroloquinoline-quinone), glucose dehydrogenase Amano 5, m-GDH, membrane glucose dehydrogenase, membrane-bound glucose dehydrogenase, membrane-bound PQQ-dependent glucose dehydrogenase, mGDH, PQQ GDH, PQQ glucose dehydrogenase, PQQ-dependent GDH, PQQ-dependent glucose dehydrogenase, PQQ-dependent mGDH, PQQ-dependent soluble glucose dehydrogenase, PQQ-GDH, PQQ-glucose dehydrogenase, PQQ-linked GCD, PQQ-sGDH, PqqC, PQQGDH, PQQGDH-B, pyrroloquinoline quinone dependent glucose dehydrogenase, pyrroloquinoline quinone glucose dehydrogenase, pyrroloquinoline quinone-dependent glucose dehydrogenase, QGDH, quinone-dependent glucose dehydrogenase, quinoprotein aldose sugar dehydrogenase, quinoprotein D-glucose dehydrogenase, quinoprotein glucose dehydrogenase, quinoprotein glucose DH, s-GDH, sGDH, sGDH-PQQ, soluble glucose dehydrogenase, soluble PQQ-dependent glucose dehydrogenase, soluble quinoprotein (PQQ-containing) glucose dehydrogenase, Tt_ASD, water-soluble PQQ glucose dehydrogenase, water-soluble pyrroquinoline quinone glucose dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.5 With a quinone or similar compound as acceptor
                1.1.5.2 glucose 1-dehydrogenase (PQQ, quinone)

Purification

Purification on EC 1.1.5.2 - glucose 1-dehydrogenase (PQQ, quinone)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Toyopearl column chromatography and hydroxyapatite column chromatography
-
HiPrep 16/10 SP XL column chromatography
HisTrap column chromatography, gel filtration
-
linked-dimeric enzyme
-
mutant enzymes
-
Ni-NTA column chromatography
-
optimization of the purification process involving cation exchange chromatography in presence of Zn2+
QAE-Toyopearl column chromatography, CM-Toyopearl column chromatography, and Sephacryl S-200 gel filtration
-
recombinant cytochrome c fusion protein from Escherichia coli
-
recombinant isozyme PQQGDH-B from Pichia pastoris culture medium, optimization of down-stream processing
-
recombinant soluble His-tagged enzyme 11.3fold from Escherichia coli strain BL21 (DE3)by nickel affinity chromatography
-
recombinant Strep-tagged membrane-bound glucose dehydrogenase from from membranes of the expressing Gluconobacter oxydans strain by streptactin affinity chromatography to homogeneity
-
soluble enzyme and membrane-bound enzyme
-
Talon column chromatography
-
the enzyme is purified (not further specified). The enzyme stock solution has an activity of 64 U/ml and a concentration of 20 mg protein/ml. For the GDH immobilization, 2 ml of the enzyme stock solution in the 0.05 M phosphate buffer (pH 6) are applied onto the active area of the Prussian Blue-modified graphite electrode. After air-drying the electrode is incubated for 10 min in glutaraldehyde vapor 25% solution, and then kept in refrigerator
-
Triton X-114 phase precipitation, acetone precipitation, polyethylene glcyol 6000 precipitation, ethanol precipitation, and hydroxylapatite column chromatography
-
using a His Microspin purification module
using a Ni-NTA column, the hexahistidine tag is removed by cleavage with tobacco edge virus protease, cleaved protein is purified by gel filtration
-
using two column chromatographies of DEAE-Toyopearl and ceramic hydroxyapatite
-
wild-type and mutant enzyme H262Y
-