1.1.5.10: D-2-hydroxyacid dehydrogenase (quinone)

This is an abbreviated version!
For detailed information about D-2-hydroxyacid dehydrogenase (quinone), go to the full flat file.

Reaction

Synonyms

(R)-2-hydroxy acid dehydrogenase, (R)-2-hydroxy-acid:(acceptor) 2-oxidoreductase, D-lactate dehydrogenase, DLD, Fe-S D-iLDH, lldE, NAD-independent D-lactate dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.5 With a quinone or similar compound as acceptor

                1.1.5.10 D-2-hydroxyacid dehydrogenase (quinone)

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Results	in table
2	AA Sequence
2	Cloned(Commentary)
5	Cofactor
2	General Information
16	Inhibitors
6	kcat/KM [mM/s]
6	KM Value [mM]
2	Localization
1	Metals/Ions
3	Molecular Weight [Da]
5	Organism
1	pH Optimum
4	pH Stability
3	Purification (Commentary)
1	Reaction
5	Reference
2	Source Tissue
2	Storage Stability
10	Substrates and Products (Substrate)
1	Subunits
8	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Stability [°C]
6	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.1.5.10 - D-2-hydroxyacid dehydrogenase (quinone)

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

D-2-hydroxybutyrate + ubiquinone-10

2-oxobutyrate + ubiquinol-10

Pseudomonas putida

Q88DT2

-

763205

-

-

?

D-glycerate + ubiquinone-10

? + ubiquinol-10

Pseudomonas putida

Q88DT2

-

763205

-

-

?

D-lactate + 2,6-dichlorophenol-indophenol

pyruvate + reduced 2,6-dichlorophenol-indophenol

Oryctolagus cuniculus

-

ordered mechanism, first D-lactate and then 2,6-dichlorophenol-indophenol bind to the enzyme to form a ternary complex, from which pyruvate and reduced 2,6-dichlorophenol-indophenol dissociate in that oder

726894

-

-

?

D-lactate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

pyruvate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

Pseudomonas putida

Q88DT2

-

763205

-

-

?

D-lactate + oxidized 2,6-dichloroindophenol

pyruvate + reduced 2,6-dichloroindophenol

Oryctolagus cuniculus

-

-

724205

-

-

?

D-lactate + ubiquinone

pyruvate + ubiquinol

Oryctolagus cuniculus

-

the rate with 0.06 mM ubiquinone is 72% of the rate with 2,6-dichloroindophenol

724205

-

-

?

D-lactate + ubiquinone-10

pyruvate + ubiquinol-10

Pseudomonas putida

Q88DT2

-

763205

-

-

?

L-lactate + 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

pyruvate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

Pseudomonas putida

Q88DT2

-

763205

-

-

?

L-lactate + ubiquinone-10

pyruvate + ubiquinol-10

Pseudomonas putida

Q88DT2

-

763205

-

-

?

additional information

?

-

Pseudomonas putida

Q88DT2

the enzyme exhibits narrow substrate specificity. No activity with D-2-hydroxybutyrate, L-2-hydroxybutyrate, D-mandelate, L-mandelate, D-3-phenyllactate, D-glycerate, L-glycerate, DL-2-hydroxyisocaproate, and DL-2-hydroxyoctanoate, with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as an electron acceptor

763205

-

-

-