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1.1.3.6: cholesterol oxidase

This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.

Word Map on EC 1.1.3.6

Reaction

cholesterol
+
O2
=
cholest-5-en-3-one
+
H2O2

Synonyms

3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.6 cholesterol oxidase

Cloned

Cloned on EC 1.1.3.6 - cholesterol oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ChoL, a fragment of a cholesterol oxidase is expressed in Escherichia coli BL21(DE3) plysS cells
cholesterol oxidase II, expression in Escherichia coli MM294/pnH10
-
cloning, sequencing and analysis of partial segment of cholesterol oxidase, the pTZ57R/T vector is used for molecular cloning
construction of a constitutive high-level-expression vector for the genus Bifidobacterium to express cholesterol oxidase from Streptomyces coelicola. The promoter region of the 16S rRNA gene is amplified by inverse PCR and used for the construction of pBES16PR. The optimal ribosome-binding site for Bifidobacterium is incorporated in pBES16PR. pBES16PR-CHOL with the structural gene for cholesterol oxidase under the control of the 16S rRNA promoter is used to transform Bifidobacterium longum. The gene is successfully expressed and high level of cholesterol oxidase activity is obtained in Bifidobacterium longum
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expression in Escherichia coli
expression in Escherichia coli strain BL21-CodonPlus (DE3)-RP of the enzyme with and without N- or C-terminal His-tags, the hydrophobicity of the enzyme is reduced by His-tags
-
expression in Escherichia coli, His-tagged enzyme
-
expression in Escherichia coli. Applying the optimized protocol, the production of recombinant cholesterol oxidase is significantly enhanced from 3.2 to 158 U/l. Under the optimized condition, the enzyme is produced on a large-scale
expression in Streptomyces lividans
-
expression of wild-type and mutant enzymes with with His-tags both at the N-terminus and at the C-terminus in Escherichia coli strain BL21 (DE3)
-
gene choF, DNA and amino acid sequence determination and analysis, expression in Escherichia coli and Pichia pastoris
gene choM, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged isozyme in Bacillus subtilis strain 168/pMA5 mainly intracellularly
gene choM1, DNA and amino acid sequence determination and analysis, genetic organization, expression of His-tagged isozyme ChoM1 in Escherichia coli strain BL21(DE3), subcloning in Escherichia cli strain DH5alpha, overexpression of isozyme ChoM1 in Mycobacterium neoaurum strain NwIB-01MS
gene choM2, DNA and amino acid sequence determination and analysis, genetic organization, expression of full-length isozyme ChoM2 and of the isozyme without the putative signal peptide sequence in Escherichia coli strain BL21(DE3), subcloning in Escherichia cli strain DH5alpha, overexpression of isozyme ChoM2 in Mycobacterium neoaurum strain NwIB-R10
gene choP, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant overexpression in Escherichia coli strain Rosetta (DE3) pLysS in inclusion bodies from cells grown at 20°C and 30°C. A soluble and active PA157 enzyme is obtained when the recombinant cells were grown at 10°C
gene HMPREF0204_11499, phylogenetic tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain JM109. Only when the cultivation temperature is decreased to 16°C immediately after induction, soluble and active protein is present
overexpression in Escherichia coli
recombinant expression of His6-tagged enzyme in Escherichia coli train BL21(DE3)
the cholesterol oxidase gene, cloned in the vector pET-28a, is overexpressed in Escherichia coli BL21-CodonPlus