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1.1.3.4: glucose oxidase

This is an abbreviated version!
For detailed information about glucose oxidase, go to the full flat file.

Word Map on EC 1.1.3.4

Reaction

beta-D-glucose
+
O2
=
D-glucono-1,5-lactone
+
H2O2

Synonyms

AldO, beta-D-glucose oxidase, beta-D-glucose oxygen-1-oxidoreductase, beta-D-glucose/oxygen 1-oxidoreductase, beta-D-glucose: oxygen 1-oxidoreductase, beta-D-glucose:O2 1-oxidoreductase, beta-D-glucose:O2-1-oxidoreductase, beta-D-glucose:oxygen 1-oxido-reductase, beta-D-glucose:oxygen 1-oxidoreductase, beta-D-glucose:oxygen oxidoreductase, beta-D-glucose:oxygen-1-oxidoreductase, beta-D-glucose:quinone oxidoreductase, CngoxA, corylophyline, D-glucose oxidase, D-glucose-1-oxidase, deoxin-1, glucose aerodehydrogenase, glucose oxyhydrase, glucose-1-oxidase, GO-2, GOD, GOX, GOxP5, microcid, More, notatin, oxidase, glucose, pen-GOx, penatin, yGOXpenag

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.4 glucose oxidase

Temperature Stability

Temperature Stability on EC 1.1.3.4 - glucose oxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 70
-
the enzyme shows a pH-dependent response to the high pressure homogenization treatment, with reduction or maintenance of activity at pH 4.5-6.0 and a remarkable activity increase (30-300%) at pH 6.5 at all tested temperatures. i.e. 15°C, 50°C and 75°C. The enzyme's thermal tolerance is reduced due to high pressure homogenization treatment and the storage for 24 h at high temperatures of 50°C and 75°C also causes a reduction of activity
20
-
half-life: 29.64h
20 - 40
50% of enzyme activity is maintained after 90 min incubation at 20-40°C
20 - 60
the activity of recombinant GOD increases slightly at the reaction temperature ranging from 20-40°C, and then falls moderately till 60°C. The recombinant enzyme retains more than 90% of activity within 40°C. Over 30% of activity is lost at 50°C, whereas almost no activity is detected above 60°C
25
-
10 h, purified enzyme, stable
25 - 70
-
70% relative activity after 30 min at 25°C, 100% activity after 30 min at 30°C, 90% relative activity after 30 min at 37°C, 75% relative activity after 30 min at 50°C, 30% relative activity after 30 min at 70°C
30 - 50
purified recombinant free enzyme and immobilized enzyme, the enzyme activity remains rather unchanged from 30-45°C and then drops at higher temperatures. The thermal stability of immobilized GOx is only slightly higher than that of the free enzyme
4 - 54
-
1 h, the enzyme retains at least 80% activity
40 - 60
purified recombinant enzyme, more than 90% activity remaining after incubation for 30 min at 50°C and 54% at temperatures up to 55°C
45 - 65
-
at 45°C the enzyme half-life is 99 min and at 65°C it is 20.38 min under similar conditions
47
-
after 6 h of incubation the percentage of activity is 68%
50 - 60
-
at pH 6.0 and at 50°C, the half life of the enzyme is about 20 h, thermal denaturation is observed above 60°C
50 - 70
-
the half-life is diminished from 210 min at 50°C to 0.61 min at 70°C, the inactivation rate constant decreases by up to 50% at temperatures between 50 and 70°C in the presence of 0.6 M trehalose
50.6
Tm-value of wild-type enzyme: 50.6°C
54
-
after 6 h of incubation the percentage of activity is 11%
55.8
-
transition temperature is independent of the protein concentration. The thermally denatured enzyme is a compact structure, a form of molten globule-like apoenzyme
56
-
half-life of native enzyme without additive: 86 min, half-life of enzyme in presence of lysozyme: 322 min, half-life of enzyme in presence of 1 M NaCl: 1806 min, half-life of enzyme in presence of 0.2 M K2SO4: 1446 min
58.6
Tm-value of mutant enzyme Y169C/A211C
59
-
midpoint for thermal inactivation of residual activity and dissocation of FAD
62
-
midpoint for loss of secondary and tertiary structure
63
-
half-life of native enzyme without additive: 7.5 min, half-life of enzyme in presence of lysozyme: 24 min, half-life of enzyme in presence of 1 M NaCl: 146 min, half-life of enzyme in presence of 0.2 M K2SO4: 62 min
67
-
half-life of native enzyme without additive: 4.5 min, half-life of enzyme in presence of lysozyme: 12 min, half-life of enzyme in presence of 1 M NaCl: 58 min, half-life of enzyme in presence of 0.2 M K2SO4: 27.5 min
72.4
-
denaturation point of native enzyme
72.8
-
denaturation point of periodate-oxidized enzyme
73
-
70% loss of activity after 60 min
75
purified recombinant free enzyme, loss of 50% activity
additional information