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1.1.3.4: glucose oxidase

This is an abbreviated version!
For detailed information about glucose oxidase, go to the full flat file.

Word Map on EC 1.1.3.4

Reaction

beta-D-glucose
+
O2
=
D-glucono-1,5-lactone
+
H2O2

Synonyms

AldO, beta-D-glucose oxidase, beta-D-glucose oxygen-1-oxidoreductase, beta-D-glucose/oxygen 1-oxidoreductase, beta-D-glucose: oxygen 1-oxidoreductase, beta-D-glucose:O2 1-oxidoreductase, beta-D-glucose:O2-1-oxidoreductase, beta-D-glucose:oxygen 1-oxido-reductase, beta-D-glucose:oxygen 1-oxidoreductase, beta-D-glucose:oxygen oxidoreductase, beta-D-glucose:oxygen-1-oxidoreductase, beta-D-glucose:quinone oxidoreductase, CngoxA, corylophyline, D-glucose oxidase, D-glucose-1-oxidase, deoxin-1, glucose aerodehydrogenase, glucose oxyhydrase, glucose-1-oxidase, GO-2, GOD, GOX, GOxP5, microcid, More, notatin, oxidase, glucose, pen-GOx, penatin, yGOXpenag

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.4 glucose oxidase

Cloned

Cloned on EC 1.1.3.4 - glucose oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the gene encoding the enzyme and expression in Escherichia coli
-
construction of a recombinant strain, the recombinant strain produces up to four times more extracellular enzyme than wild type under identical conditions
-
expressed in Nicotiana tabaccum cultivar SR1
-
expressed in Pichia pastoris
expressed in Pichia pastoris KM71H
expressed in Pichia pastoris strain GS115
expressed in Pichia pastoris strain SMD1168
expressed in Saccharomyces cerevisiae
-
expression in Escherichia coli, expression of all enzyme variants leads to the formation of insoluble inclusion bodies. Refolding of most variants can be followed by measuring increases in enzyme activity
expression in Pichia pastoris GS115
expression in Pichia pastoris X33
expression in Pichia pastoris. Cloned into the expression vector, pPIC9 and screened by the alcohol oxidase promoter. The enzyme production increases at 28°C. Enzyme activity induced by 1.0% methanol and the highest level of enzyme production is the result of shaking rate at 225 rpm
expression in Saccharomyces cerevisiae mutant deficient in PMR1 gene. GOD-His6 expressed in wild-type strain is hyperglycosylated, GOD-His6 expressed in pmr1DELTA strain is not hyperglycosylated
-
expression in Yarrowia lipolytica. Yarrowia lipolytica is a suitable and efficient eukaryotic expression system to production of recombinant enzyme (GOX) and can be used to production of pure form of GOX for industrial applications
expression of wild-type and mutant enzyme in Pichia pastoris
gene GOX, DNA sequence determination and analysis, expression in a larger scale in Pichia pastoris strain X33 using the methanol inducible AOX1 promoter, the enzyme is secreted
gene gox, recombinant expression of the extracellular enzyme in Pichia pastoris strain GS115
gene gox, recombinant expression of wild-type and M12 mutant enzymes in Pichia pastoris strain KM71H or in Saccharomyces cerevisiae strain InvSc1
gene HaGox, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real time-PCR expression analysis
isolation of the gene encoding the enzyme, DNA sequence analysis of the coding region shows 80% identity to the sequence of a enzyme gene previously published
-
overexpressed either in Escherichia coli or in Pichia pastoris
overexpression in Saccharomyces cerevisiae
recombinant enzyme expression in the periplasm or on the cell surface of Escherichia coli cells of different strains. The enzyme is differently tagged, i.e. expressed as Tat-AldO or INP-AldO, and exported to the periplasm or to the cell surface of the transformed cells, overview. AldO is successfully displayed at the surface of E. coli using a truncated INP variant, it contains covalently bound FAD, thus has attained a correctly folded and active conformation
-
recombinant expression of His-tagged enzyme in Pichia pastoris strain KM71H
recombinant expression of mutant enzyme B11 in Saccharomyces cerevisiae strain EBY100 cell wall
recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain BY4741 (MATa his3 leu2 met15 ura3) from plasmid pSSP-GOX, the GOX coding sequence is fused to the STA1 signal peptide from Saccharomyces cerevisiae var. diastaticus and is under the control of the galactose-inducible GAL10/CYC1 promoter
recombinant protein, expressed in Escherichia coli strain BL21, pET17b expression vector
-