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1.1.3.17: choline oxidase

This is an abbreviated version!
For detailed information about choline oxidase, go to the full flat file.

Word Map on EC 1.1.3.17

Reaction

Betaine aldehyde
+
O2
+
H2O
=
betaine
+
H2O2

Synonyms

alkaliphilic choline oxidase, ANI01nite_22550, An_CodA, APChO-syn, CHO, choline oxidase, choline-oxygen 1-oxidoreductase, choline:oxygen 1-reductase, ChOx, ChOx protein, codA, COX

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.17 choline oxidase

Temperature Stability

Temperature Stability on EC 1.1.3.17 - choline oxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
wild-type, stable for at least 3 h
32
melting temperature of wild-type
40
mutant S101A/V355T/F357R/M359R, 3 h, 10% residual activity. Mutant S101A/D250G/F253R/V355T/F357R/M359R, 3 h, almost 50% residual activity
40 - 45
-
incubated for 10 min at pH 8.5 and 40°C 20% initial activity is lost, 96% at 45°C, only 10% initial activity is lost at 45°C in presence of 10% glycerol
52
metling temperature of mutant S101A/D250G/F253R/V355T/F357R/M359R
70 - 90
-
no degradation when incubated for 1 h
additional information