1.1.3.13: alcohol oxidase

This is an abbreviated version!
For detailed information about alcohol oxidase, go to the full flat file.

Reaction

Synonyms

alcohol oxidase 1, alcohol oxidase 2, alcohol oxidase A, alcohol oxidase B, alcohol oxidase I, alcohol: O2 oxidoreductase, alcohol:dioxygen-oxidoreductase, alcohol:O2 oxidoreductase, AlcOx, AOd, AOD1, AOext, AOint, AOX, AOX1, AOX2, AOXI, broad substrate specific alcohol oxidase, EC 1.1.3.31, ethanol oxidase, extracellular alcohol oxidase, FAD-dependent alcohol oxidase, FAO1, GLRG_05590, intracellular alcohol oxidase, long chain fatty alcohol oxidase, methanol oxidase, Mod1p, Mod2p, oxidase, alcohol, P-AOD, peroxisomal alcohol oxidase, primary alcohol oxidase, SCAO, short chain alcohol oxidase, short-chain alcohol oxidase, VAO, veratryl alcohol oxidase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.3 With oxygen as acceptor

                1.1.3.13 alcohol oxidase

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Results	in table
9	Activating Compound
107	AA Sequence
38	Application
1	CAS Registry Number
13	Cloned(Commentary)
52	Cofactor
6	Crystallization (Commentary)
1	Expression
3	General Information
13	General Stability
145	Inhibitors
38	kcat/KM [mM/s]
8	Ki Value [mM]
147	KM Value [mM]
35	Localization
6	Metals/Ions
57	Molecular Weight [Da]
86	Natural Substrates/ Products (Substrates)
18	Organic Solvent Stability
184	Organism
6	Pathway
29	PDB ID
36	pH Optimum
13	pH Range
28	pH Stability
10	pI Value
3	Posttranslational Modification
21	Protein Variants
35	Purification (Commentary)
2	Reaction
3	Reaction Type
146	Reference
1	Renatured (Commentary)
44	Source Tissue
55	Specific Activity [micromol/min/mg]
17	Storage Stability
476	Substrates and Products (Substrate)
49	Subunits
108	Synonyms
1	Systematic Name
27	Temperature Optimum [°C]
5	Temperature Range [°C]
55	Temperature Stability [°C]
47	Turnover Number [1/s]

pH Stability

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pH Stability on EC 1.1.3.13 - alcohol oxidase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

4.2

Komagataella pastoris

-

purified recombinant His-tagged enzyme, 25°C, 3 h, inactivation

724630

5

Basidiomycota

-

25°C, 3 h, complete inactivation

484905

5 - 10

Paecilomyces variotii

-

stable

684630

5 - 11

Thermoascus aurantiacus

-

purified intracellular enzyme, stable

674961

5 - 6

Ochrobactrum sp. AIU 033

-

20 min, 25°C, more than 80% residual activity, recombinant protein

763283

5.5 - 7

Komagataella pastoris

-

purified recombinant His-tagged enzyme, 25°C, 3 h, 50% activity remaining

724630

5.5 - 8.5

Ogataea angusta

-

stable

484914

6

3 entries

6 - 11

Thermoascus aurantiacus

-

purified extracellular enzyme, stable

674961

6 - 8

Candida sp. (in: Saccharomycetales)

-

30 min, 45 min, stable

484907

6 - 9

Aspergillus terreus

-

stable at pH 9.0, loss of 60% activity at pH 6.0, the activity is drastically reduced at below neutral pH and above pH 9.0, overview

671539

6.5

2 entries

6.5 - 8.5

2 entries

7

Phlebiopsis gigantea

-

22°C, 50% loss of activity after 60 min

484922

7 - 10

[Candida] boidinii

-

stable

484912

7 - 11

Gloeophyllum trabeum

A8DPS4

pH stability optimum on the alkaline side in the range of pH 7 to 11. 80% of maximum stability at pH 9

684565

7 - 8

Komagataella pastoris

-

stable

484910

7 - 9

Basidiomycota

-

25°C, 3 h, stable

484905

8

Phlebiopsis gigantea

-

22°C, 50% loss of activity after 28 h

484922

8 - 10

2 entries

9

3 entries

6

Aspergillus ochraceus

-

40°C, 20 min, about 60% loss of activity

688123

6

Penicillium purpurescens

-

the enzyme activity is enhanced to 2fold by incubation at pH 6.0 and 40°C for 60 min, whereas the Km values for ethanol and ethylene glycol do not change

699050

6

Phlebiopsis gigantea

-

22°C, 50% loss of activity after 10 min

484922

6.5

Basidiomycota

-

-

484906

6.5

Basidiomycota

-

rapid inactivation below

484905

6.5 - 8.5

Aspergillus ochraceus

-

40°C, 20 min, more than 90% of the activity remains

688123

6.5 - 8.5

[Candida] boidinii

-

stable

484916

8 - 10

Komagataella pastoris

-

purified recombinant His-tagged enzyme, 25°C, 3 h, stable

724630

8 - 10

Ogataea angusta

-

stable

484910

9

Aspergillus terreus

-

3 h, 5% loss of activity

685404

9

Komagataella pastoris

-

and above, inactivation

484910

9

Phlebiopsis gigantea

-

22°C, 50% loss of activity after 10 min

484922