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1.1.3.13: alcohol oxidase

This is an abbreviated version!
For detailed information about alcohol oxidase, go to the full flat file.

Word Map on EC 1.1.3.13

Reaction

a primary alcohol
+
O2
=
an aldehyde
+
H2O2

Synonyms

alcohol oxidase 1, alcohol oxidase 2, alcohol oxidase A, alcohol oxidase B, alcohol oxidase I, alcohol: O2 oxidoreductase, alcohol:dioxygen-oxidoreductase, alcohol:O2 oxidoreductase, AlcOx, AOd, AOD1, AOext, AOint, AOX, AOX1, AOX2, AOXI, broad substrate specific alcohol oxidase, EC 1.1.3.31, ethanol oxidase, extracellular alcohol oxidase, FAD-dependent alcohol oxidase, FAO1, GLRG_05590, intracellular alcohol oxidase, long chain fatty alcohol oxidase, methanol oxidase, Mod1p, Mod2p, oxidase, alcohol, P-AOD, peroxisomal alcohol oxidase, primary alcohol oxidase, SCAO, short chain alcohol oxidase, short-chain alcohol oxidase, VAO, veratryl alcohol oxidase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.13 alcohol oxidase

Storage Stability

Storage Stability on EC 1.1.3.13 - alcohol oxidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20 to -80°C, 0.02 M phosphate-citrate buffer, pH 8-9, in presence of 30-50% (w/v) sucrose, 4 years, no loss of activity
Pichia putida
-
-20°C, stable
-
0°C, stable at pH 6.5-8.5
-
25°C, purified free enzyme, 10 weeks, 20% remaining actiivty
-
25°C, purified immobilized enzyme, 10 weeks, 60% remaining actiivty
-
4°C, 1 month, more than 50% of the activity is retained
-
4°C, 1 week storage of the sterile-filtered purified enzyme in 20 mM sodium phosphate (pH 7.5) results in a 68% loss of activity
4°C, lyophilized, 20% loss of activity within a year
-
4°C, purified enzyme, 24 h, loss of only 5% of activity
-
4°C, purified free enzyme, 10 weeks, 70% remaining actiivty
-
4°C, purified immobilized enzyme, 10 weeks, 90% remaining actiivty
-
4°C, purified, stable for 2 weeks
4°C, stable for at least 6 months
-
4°C, storage in presence of antimicrobial agent sodium azide, 0.01% in 50 mM potassium phosphate buffer, pH 7.7, stable for at least 2 weeks
Poria contigua
-
crystalline, stable for at least 5 months in 0.05 M phosphate buffer, pH 7.7
-
the immobilized enzyme shows a high stability against long storage
-