1.1.1.B52: 3-quinuclidinone reductase (NADH)

This is an abbreviated version, for detailed information about 3-quinuclidinone reductase (NADH), go to the full flat file.

Reaction

(R)-3-quinuclidinol
+
NAD+
=
3-quinuclidinone
+
NADH
+
H+

Synonyms

3-quinuclidinone reductase, alcohol dehydrogenase, ArQR, AtQR, BacC, NADHdependent 3-quinuclidionone reductase, QNR

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B52 3-quinuclidinone reductase (NADH)

Purification

Purification on EC 1.1.1.B52 - 3-quinuclidinone reductase (NADH)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; 3-quinuclidinone reductase QNR
-
native enzyme by anion exchange chromatography, dialysis, hydrophobic interaction chromatography, and dialysis, recombinant His-tagged enzyme from Escherichia coli 1.7fold by nickel affinity chromatography and ultrafiltration; native enzyme by anion exchange chromatography, dialysis, hydrophobic interaction chromatography, and dialysis, recombinant His-tagged enzyme from Escherichia coli 2.4fold by nickel affinity chromatography and ultrafiltration
recombinant N-terminally His6-tagged enzyme from Eschrichia coli strain Rosetta(DE3) by nickel affinity and anion exchange chromatography, and gel filtration
-