1.1.1.B52: 3-quinuclidinone reductase (NADH)

This is an abbreviated version, for detailed information about 3-quinuclidinone reductase (NADH), go to the full flat file.

Reaction

(R)-3-quinuclidinol
+
NAD+
=
3-quinuclidinone
+
NADH
+
H+

Synonyms

alcohol dehydrogenase, ArQR, AtQR, BacC, QNR

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B52 3-quinuclidinone reductase (NADH)

Engineering

Engineering on EC 1.1.1.B52 - 3-quinuclidinone reductase (NADH)

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D40A
-
no activity
E197A
-
no activity
R196A
-
activity is 76% compared to activity of wild-type enzyme
Y216V
-
activity is 31% compared to activity of wild-type enzyme
A158A/N162P
-
96.8% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion
A158D/N162G
-
96.5% enantiomeric excess for (R)-3-quinuclidinol production, 87% conversion
A158D/N162Q
-
95.8% enantiomeric excess for (R)-3-quinuclidinol production, 79% conversion
A158H/N162G/K202Q/L224W
-
mutations increase the enantiomeric excess for (R)-3-quinuclidinol production from 84.3% (wild-type) to 99% and concomitantly to enhance conversion by 43.5%
A158H/N162P
-
98.1% enantiomeric excess for (R)-3-quinuclidinol production, 95% conversion
A158K/N162M
-
97.6% enantiomeric excess for (R)-3-quinuclidinol production, 98% conversion
K202N/L224M
-
89.7% enantiomeric excess for (R)-3-quinuclidinol production, 90% conversion
K202Q/L224W
-
95.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion
K202R/L224W
-
94.7% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion
K202S/L224Y
-
89.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion
N162A
-
93.8% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion