1.1.1.B40: 11beta-hydroxysteroid dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about 11beta-hydroxysteroid dehydrogenase (NAD+), go to the full flat file.

Reaction

an 11beta-hydroxysteroid
+
NAD+
=
an 11-oxosteroid
+
NADH
+
H+

Synonyms

11 beta-hydroxysteroid dehydrogenase, 11 beta-hydroxysteroid dehydrogenase type 2, 11-beta-HSD2, 11beta hydroxysteroid dehydrogenase type 2, 11beta-HSD, 11beta-HSD 2, 11beta-HSD type 2, 11beta-HSD1, 11beta-HSD2, 11beta-HSD2 (oxidase) NAD+, 11beta-hydroxysteroid dehydrogenase 2, 11beta-hydroxysteroid dehydrogenase isoforms 2, 11beta-hydroxysteroid dehydrogenase type 1, 11beta-hydroxysteroid dehydrogenase type 2, 11beta-hydroxysteroid dehydrogenase type-2, 11beta-hydroxysteroid dehydrogenases type 2, 11beta-OHSD, 11betaHSD2, 7-alpha-HCD, 7-alpha-hydroxycholesterol dehydrogenase, corticosteroid 11-beta-dehydrogenase isozyme 1, HSD 2, isozyme 11beta-HSD2, More, NAD(+)-dependent 11beta-dehydrogenase, NAD+-dependent 11beta-hydroxysteroid dehydrogenase 2, type 2 11beta-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B40 11beta-hydroxysteroid dehydrogenase (NAD+)

Crystallization

Crystallization on EC 1.1.1.B40 - 11beta-hydroxysteroid dehydrogenase (NAD+)

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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, 25 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 0.1& Triton, 5% glycerol, and 2 mM EDTA, mixed with equal volume of well solution containing 1.8 M Li2O4, and 0.1 M HEOES, pH 7.5, at room temperature, derivatization with 1 mM ethylmercuric chloride, soaking of crystals with NADH and corticoserone containing solution, X-ray diffraction structure determination and analysis at 3.0 A resolution
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