1.1.1.B4: (R)-specific secondary alcohol dehydrogenase

This is an abbreviated version, for detailed information about (R)-specific secondary alcohol dehydrogenase, go to the full flat file.

Reaction

(R)-R-CHOH-R'
+
NAD+
=
R-CO-R'
+
NADH
+
H+

Synonyms

ADH, ADH1, ADH2, ADHTt, carbonyl reductase (NADH, specific for (S)-configuration of alcohol), CHY1186, More, SDR, short-chain NAD(H)-dependent alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B4 (R)-specific secondary alcohol dehydrogenase

Purification

Purification on EC 1.1.1.B4 - (R)-specific secondary alcohol dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His-tagged mutant I86A from Escherichia coli HB101 (DE3) by nickel affinity chromatography
recombinant enzyme 11fold to homogeneity from Escherichia coli strain BL21(DE3) by heat denaturation of host proteins, anion exchange chromatography and gel filtration
-