1.1.1.B4: (R)-specific secondary alcohol dehydrogenase

This is an abbreviated version, for detailed information about (R)-specific secondary alcohol dehydrogenase, go to the full flat file.

Reaction

(R)-R-CHOH-R'
+
NAD+
=
R-CO-R'
+
NADH
+
H+

Synonyms

ADH, ADH1, ADH2, ADHTt, carbonyl reductase (NADH, specific for (S)-configuration of alcohol), CHY1186, More, SDR, short-chain NAD(H)-dependent alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B4 (R)-specific secondary alcohol dehydrogenase

Engineering

Engineering on EC 1.1.1.B4 - (R)-specific secondary alcohol dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A90S
-
activity with NADPH as cofactor decreased to about 50% of wild-type, activity with NADH strongly decreased
G37D/R38P
-
activity with NADH is decreased relative to Arg38Pro alone, but is higher than that of the wild-type enzyme
M140I
-
no activity with NADPH as cofactor
R38P
-
no activity with NADPH as cofactor, fourfold increase in activity with NADH
V112D
-
no activity with NADPH as cofactor, strongly decreased activity with NADH
W95L/N249Y
-
the mutant exhibits higher activity but decreased affinity toward aliphatic alcohols, aldehydes as well as NAD+ and NADH compared to the wild type enzyme, optimum pH is at about pH 8.6
I86A
site-directed mutagensis, the secondary alcohol dehydrogenase I86A mutant is stereospecific for (R)-alcohols instead of (S)-alcohols, in contrast to the wild-type enzyme, the mutation I86A allows large substituents to fit into the large pocket of I86ATeSADH, which corresponds to the small pocket in wild-type TeSADH, modeling of the stereopreference of TeSADH I86A