1.1.1.B4: (R)-specific secondary alcohol dehydrogenase

This is an abbreviated version, for detailed information about (R)-specific secondary alcohol dehydrogenase, go to the full flat file.

Reaction

(R)-R-CHOH-R'
+
NAD+
=
R-CO-R'
+
NADH
+
H+

Synonyms

ADH, ADH1, ADH2, ADHTt, carbonyl reductase (NADH, specific for (S)-configuration of alcohol), CHY1186, More, SDR, short-chain NAD(H)-dependent alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B4 (R)-specific secondary alcohol dehydrogenase

Crystallization

Crystallization on EC 1.1.1.B4 - (R)-specific secondary alcohol dehydrogenase

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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant His-tagged mutant I86A, hanging drop vapour diffusion, mixing of 0.002 ml of 8 mg/ml protein in 50 mM 2-(N-morpholino)ethanesulfonic acid, pH 6.6, 20 mM NaCl, 0.1 mM dithiothreitol, 0.1 mM ethylenediaminetetraacetic acid, and 2 mM (R)-1-phenylethanol, with 0.2% D-fructose 1,6-disphosphate trisodium salt octahydrate, 0.2% glycerol phosphate disodium salt hydrate, 0.2% O-phospho-L-serine, 0.2% O-phosphol-L-styrosine, 0.2% phytic acid sodium salt hydrate, 0.02 M HEPES sodium pH 6.8, and 0.002 ml reservoir solution containing 20% v/v PEG 400, 0.1 M Na HEPES, pH 7.0, room temperature, 6-12 h, X-ray diffraction structure determination and analysis, molecular replacement, modeling