1.1.1.B20: meso-2,3-butandiol dehydrogenase

This is an abbreviated version, for detailed information about meso-2,3-butandiol dehydrogenase, go to the full flat file.

Reaction

(2R,3S)-butane-2,3-diol
+
NAD+
=
acetoin
+
NADH
+
H+

Synonyms

2,3-BD dehydrogenase, 2,3-butanediol dehydrogenae, ADH-9, ARA1, BDH, budC, butACg, mbdh, meso-2,3-BD dehydrogenase, meso-2,3-BDH, meso-2,3-butanediol dehydrogenase, meso-acetoin reductase, meso-BD, meso-BDH, NAD(H)-dependent meso-2,3-BDH, NAD(H)-dependent meso-2,3-butanediol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B20 meso-2,3-butandiol dehydrogenase

Specific Activity

Specific Activity on EC 1.1.1.B20 - meso-2,3-butandiol dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.3
-
recombinant Escherichia coli strain BL21(DE3) expressing wild-type BDH, pH 7.0, 30C
1.1
-
mutant N146F, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37C
2.17
AEF50077
crude extracet of Escherichia coli cells expressing the enzyme from plasmid pET-mbdh-nox-vgb, pH 8.0, 22C
2.37
purified recombinant enzyme, pH 7.6, 37C, substrates meso-2,3-butanediol and NADP+
2.47
AEF50077
crude extracet of Escherichia coli cells expressing the enzyme from plasmid pET-mbdh-nox, pH 8.0, 22C
3.1
-
mutant Q140I/N146F, substrate (2,3S)-2,3-butanediol, pH 8.0, 37C
3.32
AEF50077
crude extracet of Escherichia coli cells expressing the enzyme from plasmid pET-mbdh, pH 8.0, 22C
4.5
-
mutant Q140I/N146F, substrate meso-2,3-butanediol, pH 8.0, 37C
5.5
-
wild-type, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37C
7.14
-
crude recombinant enzyme, pH 7.0, 37C
19.4
-
mutant N146F, substrate meso-2,3-butanediol, pH 8.0, 37C
21
-
purified reconstituted, recombinant enzyme, reduction of 4-methyl-2-pentanone, pH 8.0, 35C
66
-
purified reconstituted, recombinant enzyme, oxidation of meso-2,3-butanediol, pH 8.0, 35C
119.55
purified recombinant enzyme, pH 7.6, 37C, substrates meso-2,3-butanediol and NAD+
177
-
wild-type, substrate meso-2,3-butanediol, pH 8.0, 37C
218
-
purified reconstituted, recombinant enzyme, reduction of acetoin, pH 8.0, 35C
461.67
purified recombinant enzyme, pH 7.6, 37C, substrates acetoin and NADH