1.1.1.B20: meso-2,3-butandiol dehydrogenase

This is an abbreviated version, for detailed information about meso-2,3-butandiol dehydrogenase, go to the full flat file.

Reaction

(2R,3S)-butane-2,3-diol
+
NAD+
=
acetoin
+
NADH
+
H+

Synonyms

2,3-BD dehydrogenase, ARA1, BDH, meso-2,3-BDH, meso-2,3-butanediol dehydrogenase, meso-BD, NAD(H)-dependent meso-2,3-BDH, NAD(H)-dependent meso-2,3-butanediol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B20 meso-2,3-butandiol dehydrogenase

Engineering

Engineering on EC 1.1.1.B20 - meso-2,3-butandiol dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N146F
-
11% of wild-type specific activity
Q140I
-
trace activity below 0.1 U/mg
Q140I/N146F
-
poor activity
Q140I/N146F/W190H
-
trace activity below 0.1 U/mg with substrate meso-butanediol, 2.9 U/mg with substrate (2S,3S)-2,3-butanediol
Q140I
-
mutation mimicking the corresponding residue in (S,S)-butanediol dehydrogenase. No activity with substrates meso-butanediol or (S,S)-butanediol
Q140I/N146F
-
mutation mimicking the corresponding residues in (S,S)-butanediol dehydrogenase. Poor activity with substrates meso-butanediol or (S,S)-butanediol
additional information
-
expression of gene bdh1 from Saccharomyces cervisiae in Escherichia coi strain YYC202(DE3) ldhA-/- ilvC-/- expressing ilvBN from Escherichia coli and aldB from Lactobacillus lactis, encoding acetolactate synthase and acetolactate decarboxylase activities, respectively. Disruption of the lactate biosynthesis pathway in the strain increases pyruvate precursor availability to this strain, increased availability of NADH for acetoin reduction to meso-2,3-butanediol is the most important consequence of ldhA deletion. Optimization of 2,3-butanediol production in Escherichia coli, overview