1.1.1.95: phosphoglycerate dehydrogenase

This is an abbreviated version, for detailed information about phosphoglycerate dehydrogenase, go to the full flat file.

Reaction

3-phospho-D-glycerate
+
NAD+
=
3-phosphonooxypyruvate
+
NADH
+
H+

Synonyms

3-PGDH, 3-phosphoglycerate dehydrogenase, 3-phosphoglyceric acid dehydrogenase, 3PGDH, A10, alpha-phosphoglycerate dehydrogenase, ApPGDH, D-3-phosphoglycerate dehydrogenase, D-3-phosphoglycerate:NAD oxidoreductase, D-phosphoglycerate dehydrogenase, dehydrogenase, phosphoglycerate, EDA9, EhPGDH, glycerate 3-phosphate dehydrogenase, glycerate-1,3-phosphate dehydrogenase, More, NAD-dependent D-3-phosphoglycerate dehydrogenase, PGDH, PGDH1, PGDH2, PGDH3, PGK, Phgdh, phosphoglycerate dehydrogenase, phosphoglycerate kinase, phosphoglycerate oxidoreductase, phosphoglyceric acid dehydrogenase, serA, type 1 D-3-phosphoglycerate dehydrogenase, type III 3-phosphoglycerate dehydrogenase, type IIIK PGDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.95 phosphoglycerate dehydrogenase

Purification

Purification on EC 1.1.1.95 - phosphoglycerate dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
diverse mutants expressed in Escherichia coli
-
Ni-NTA column chromatography and gel filtration
-
Ni-NTA column chromatography; Ni-NTA column chromatography; Ni-NTA column chromatography
nickel-Sepharose column chromatography and Superdex 200 gel filtration
-
partial
-
PGDH is expressed in Escherichia coli and purified using 5'-AMP-Sepharose affinity chromatography
-
recombinant selenomethionine-labeled enzyme from strain B834
-
recombinant tagged wild-type and mutant enzymes
-
Talon cobalt based immobilized metal affinity column chromatography
using a butyl Sepharose 4 Fast Flow column and a Superdex 200 gel filtration column. During purification an unexpected decline in enzyme activity is observed if the enzyme is stored in plastic tubes, but not in glass ones
-
using ammonium sulphate fractionation and ion exchange chromatography
-