1.1.1.87: homoisocitrate dehydrogenase

This is an abbreviated version, for detailed information about homoisocitrate dehydrogenase, go to the full flat file.

Reaction

(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate
+
NAD+
=
2-oxoadipate
+
CO2
+
NADH
+
H+

Synonyms

(-)-1-hydroxy-1,2,4-butanetricarboxylate:NAD+ oxidoreductase (decarboxylating), 11-cis-RDH, 11-cis-retinol dehydrogenase, 2-hydroxy-3-carboxyadipate dehydrogenase, 3-carboxy-2-hydroxyadipate dehydrogenase, 3-carboxy-2-hydroxyadipate:NAD+ oxidoreductase (decarboxylating), atRDH, dehydrogenase, homoisocitrate, EC 1.1.1.155, HIc, HIc dehydrogenase, HICDH, homoisocitrate dehydrogenase, homoisocitric dehydrogenase, isocitrate-homoisocitrate dehydrogenase, protein PH1722, RDH10, retinol dehydrogenase 10, ScHICDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.87 homoisocitrate dehydrogenase

Purification

Purification on EC 1.1.1.87 - homoisocitrate dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purification of His-tagged enzyme using nickel affinity chromatography results in an inactive enzyme
-
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
-
recombinant enzyme from Escherichia coli strain OM17 by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain TOP 10F by nickel affinity chromatography'
-
recombinnat His-tagged enzyme from Escherichia coli by metal affinity chromatography