1.1.1.86: ketol-acid reductoisomerase (NADP+)

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For detailed information about ketol-acid reductoisomerase (NADP+), go to the full flat file.

Reaction

Synonyms

2-hydroxy-3-keto acid reductoisomerase, acetohydroxy acid isomeroreductase, acetohydroxy acid reductoisomerase, acetohydroxy-acid isomeroreductase , acetohydroxy-acid reductoisomerase , acetohydroxyacid isomeroreductase, acetolactate reductoisomerase, AHAIR, AHIR, alpha-keto-beta-hydroxylacil reductoisomerase, alpha-keto-beta-hydroxylacyl reductoisomerase, class II ketol-acid reductoisomerase, dehydrogenase, dihydroxyisovalerate (isomerizing), dihydroxyisovalerate dehydrogenase (isomerizing), EC 1.1.1.89, Icl1p, Ilv5p, ilvC, IlvC-PanE, IlvC1, IlvC2, isomerase, ketol acid reducto-, isomeroreductase, KARI, ketol-acid reductoisomerase, reductoisomerase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.86 ketol-acid reductoisomerase (NADP+)

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Results	in table
1	Activating Compound
32999	AA Sequence
7	Application
1	CAS Registry Number
24	Cloned(Commentary)
58	Cofactor
24	Crystallization (Commentary)
37	General Information
2	General Stability
222	Inhibitors
137	kcat/KM [mM/s]
77	Ki Value [mM]
225	KM Value [mM]
5	Localization
62	Metals/Ions
28	Molecular Weight [Da]
72	Natural Substrates/ Products (Substrates)
125	Organism
12	Pathway
139	PDB ID
20	pH Optimum
7	pH Range
8	pH Stability
1	pI Value
1	Posttranslational Modification
52	Protein Variants
29	Purification (Commentary)
5	Reaction
4	Reaction Type
107	Reference
1	Renatured (Commentary)
10	Source Tissue
17	Specific Activity [micromol/min/mg]
3	Storage Stability
278	Substrates and Products (Substrate)
29	Subunits
123	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
7	Temperature Stability [°C]
162	Turnover Number [1/s]

pH Range

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pH Range on EC 1.1.1.86 - ketol-acid reductoisomerase (NADP+)

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

6.5 - 8.5

Spinacia oleracea

-

pH 6.5: about 35% of maximal activity, pH 8.5: about 90% of maximal activity, reaction with 2-acetolactate, isoenzyme 1

639176

6.6 - 8.8

Neurospora crassa

-

pH 6.6: about 65% of maximal activity, pH 8.8: about 90% of maximal activity, reaction with 2-acetolactate, Tris buffer

639171

6.8 - 8

Salmonella enterica subsp. enterica serovar Typhimurium

-

pH 6.8: about 40% of maximal activity

639169

7 - 10

Corynebacterium glutamicum

Q57179

pH 7.0: 87% of maximal activity, pH 10.0: 61% of maximal activity

763172

7 - 8.5

2 entries

7 - 9

Vigna radiata var. radiata

-

pH 7.0: about 40% of maximal activity, pH 9.0: about 90% of maximal activity, with 2-hydroxy-2-ethyl-3-oxobutanoate as substrate, Tris-HCl buffer

639170

7 - 8.5

Neurospora crassa

-

pH 7.0: about 60% of maximal activity, pH 8.5: about 65% of maximal activity

639171

7 - 8.5

Spinacia oleracea

-

pH 7.0: about 25% of maximal activity, pH 8.0-8.5: optimum, isoenzyme 1

639176