1.1.1.86: ketol-acid reductoisomerase (NADP+)

This is an abbreviated version!
For detailed information about ketol-acid reductoisomerase (NADP+), go to the full flat file.

Reaction

Synonyms

2-hydroxy-3-keto acid reductoisomerase, acetohydroxy acid isomeroreductase, acetohydroxy acid reductoisomerase, acetohydroxy-acid isomeroreductase , acetohydroxy-acid reductoisomerase , acetohydroxyacid isomeroreductase, acetolactate reductoisomerase, AHAIR, AHIR, alpha-keto-beta-hydroxylacil reductoisomerase, alpha-keto-beta-hydroxylacyl reductoisomerase, class II ketol-acid reductoisomerase, dehydrogenase, dihydroxyisovalerate (isomerizing), dihydroxyisovalerate dehydrogenase (isomerizing), EC 1.1.1.89, Icl1p, Ilv5p, ilvC, IlvC-PanE, IlvC1, IlvC2, isomerase, ketol acid reducto-, isomeroreductase, KARI, ketol-acid reductoisomerase, reductoisomerase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.86 ketol-acid reductoisomerase (NADP+)

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Results	in table
1	Activating Compound
32999	AA Sequence
7	Application
1	CAS Registry Number
24	Cloned(Commentary)
58	Cofactor
24	Crystallization (Commentary)
37	General Information
2	General Stability
222	Inhibitors
137	kcat/KM [mM/s]
77	Ki Value [mM]
225	KM Value [mM]
5	Localization
62	Metals/Ions
28	Molecular Weight [Da]
72	Natural Substrates/ Products (Substrates)
125	Organism
12	Pathway
139	PDB ID
20	pH Optimum
7	pH Range
8	pH Stability
1	pI Value
1	Posttranslational Modification
52	Protein Variants
29	Purification (Commentary)
5	Reaction
4	Reaction Type
107	Reference
1	Renatured (Commentary)
10	Source Tissue
17	Specific Activity [micromol/min/mg]
3	Storage Stability
278	Substrates and Products (Substrate)
29	Subunits
123	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
7	Temperature Stability [°C]
162	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.1.1.86 - ketol-acid reductoisomerase (NADP+)

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-10°, in presence of NADPH, 5 d, 70-80% loss of activity

Neurospora crassa

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639171

-80°, 20 mM MOPS, 10% glycerol, several months, mutant enzyme DELTA423-431/F431S, no loss of activity

Spinacia oleracea

-

639185

-80°, chimeric enzyme composed of Arabidopsis thaliana acetohydroxy acid synthase genetically fused in frame with the nucleotide sequence coding for the Spinacia oleracea acetohydroxy acid isomeroreductase and expressed in E. coli, several months, no loss of activity

Spinacia oleracea

-

639184