1.1.1.86: ketol-acid reductoisomerase (NADP+)

This is an abbreviated version, for detailed information about ketol-acid reductoisomerase (NADP+), go to the full flat file.

Reaction

(2R)-2,3-dihydroxy-3-methylbutanoate
+
NADP+
=
(2S)-2-hydroxy-2-methyl-3-oxobutanoate
+
NADPH
+
H+

Synonyms

2-hydroxy-3-keto acid reductoisomerase, acetohydroxy acid isomeroreductase, acetohydroxy acid reductoisomerase, acetohydroxy-acid isomeroreductase , acetohydroxy-acid reductoisomerase , acetohydroxyacid isomeroreductase, acetolactate reductoisomerase, AHAIR, AHIR, alpha-keto-beta-hydroxylacil reductoisomerase, alpha-keto-beta-hydroxylacyl reductoisomerase, class II ketol-acid reductoisomerase, dehydrogenase, dihydroxyisovalerate (isomerizing), dihydroxyisovalerate dehydrogenase (isomerizing), Icl1p, Ilv5p, ilvC, IlvC-PanE, IlvC1, IlvC2, isomerase, ketol acid reducto-, isomeroreductase, KARI, ketol-acid reductoisomerase, reductoisomerase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.86 ketol-acid reductoisomerase (NADP+)

Purification

Purification on EC 1.1.1.86 - ketol-acid reductoisomerase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
mutant enzyme DELTA423-431/F431S; recombinant enzyme from Escherichia coli
-
Ni-NTA column chromatography
Ni-NTA-agarose resin column chromatography and Superdex 200 gel filtration
of wild-type and mutants
-
partial
recombinant enzyme from Escherichia coli
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography
-
recombinant His6-tagged enzyme
-
wild-type and mutant enzyme
-