1.1.1.86: ketol-acid reductoisomerase (NADP+)

This is an abbreviated version, for detailed information about ketol-acid reductoisomerase (NADP+), go to the full flat file.

Reaction

(2R)-2,3-dihydroxy-3-methylbutanoate
+
NADP+
=
(2S)-2-hydroxy-2-methyl-3-oxobutanoate
+
NADPH
+
H+

Synonyms

2-hydroxy-3-keto acid reductoisomerase, acetohydroxy acid isomeroreductase, acetohydroxy acid reductoisomerase, acetohydroxy-acid isomeroreductase , acetohydroxy-acid reductoisomerase , acetohydroxyacid isomeroreductase, acetolactate reductoisomerase, AHAIR, AHIR, alpha-keto-beta-hydroxylacil reductoisomerase, alpha-keto-beta-hydroxylacyl reductoisomerase, class II ketol-acid reductoisomerase, dehydrogenase, dihydroxyisovalerate (isomerizing), dihydroxyisovalerate dehydrogenase (isomerizing), Icl1p, Ilv5p, ilvC, IlvC-PanE, IlvC1, IlvC2, isomerase, ketol acid reducto-, isomeroreductase, KARI, ketol-acid reductoisomerase, reductoisomerase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.86 ketol-acid reductoisomerase (NADP+)

KM Value

KM Value on EC 1.1.1.86 - ketol-acid reductoisomerase (NADP+)

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04 - 0.24
(S)-2-acetolactate
0.01
2-aceto-2-hydroxybutanoate
-
pH 8.2, 30°C, chimeric enzyme Aabidopsis thaliana acetohydroxy acid synthase genetically fused in frame with the nucleotide sequence coding for the Spinacia oleracea acetohydroxy acid isomeroreductase and expressed in E. coli
0.002 - 0.78
2-aceto-2-hydroxybutyrate
0.01 - 5.5
2-acetolactate
0.3 - 75
2-hydroxy-2-ethyl-3-oxobutanoate
0.28 - 12
2-Hydroxy-2-methyl-3-oxobutanoate
4.56
2-Ketobutyrate
-
pH 8.0, 37°C, wild-type enzyme
6.91
2-ketoisovalerate
-
pH 8.0, 37°C, wild-type enzyme
0.17
2-ketopantoate
-
pH 8.0, 37°C, wild-type enzyme
3.15
2-Ketovalerate
-
pH 8.0, 37°C, wild-type enzyme
30 - 60
2-Methylacetoacetate
0.21
3-hydroxy-2-ketobutyrate
-
pH 8.0, 37°C, wild-type enzyme
0.29 - 50
3-hydroxy-3-ethyl-2-oxobutanoate
-
0.27
3-hydroxy-3-methyl-2-ketobutyrate
-
pH 8.0, 37°C, wild-type enzyme
0.26 - 13
3-Hydroxy-3-methyl-2-oxobutanoate
0.334 - 15.3
3-hydroxypyruvate
0.893 - 60
Hydroxypyruvate
0.019 - 0.207
NADH
0.0089 - 0.072
NADP+
0.00016 - 11.2
NADPH
1.54 - 1200
pyruvate