1.1.1.85: 3-isopropylmalate dehydrogenase

This is an abbreviated version!
For detailed information about 3-isopropylmalate dehydrogenase, go to the full flat file.

Reaction

Synonyms

2-hydroxy-4-methyl-3-carboxyvalerate:NAD+ oxidoreductase, 2R,3S-isopropylmalate:NAD+ oxidoreductase (decaboxylating), 3-IPM dehydrogenase, 3-IPM-DH, 3-isopropylmalate dehydrogenase, 3-isopropylmalateDH, APS IPMDH, beta-IPM dehydrogenase, beta-IPMDH, beta-isopropylmalate dehydrogenase, beta-isopropylmalic enzyme, Bs3-isopropylmalateDH, dehydrogenase, 3-isopropylmalate, IMDH, IPMDH, IPMDH1, IPMDH2, IPMDH3, isopropylmalate dehydrogenase, leuB, NAD-dependent isopropylmalate dehydrogenase, Saci_0600, SbIPMDH, SoIPMDH, threo-Ds-3-isopropylmalate dehydrogenase, Tt-beta-3-isopropylmalateDH, two-domain 3-isopropylmalate dehydrogenase, Ydr417cp

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.85 3-isopropylmalate dehydrogenase

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Results	in table
1	Activating Compound
36146	AA Sequence
5	Application
1	CAS Registry Number
61	Cloned(Commentary)
108	Cofactor
27	Crystallization (Commentary)
2	Expression
23	General Information
8	General Stability
44	Inhibitors
66	kcat/KM [mM/s]
2	Ki Value [mM]
293	KM Value [mM]
3	Localization
73	Metals/Ions
30	Molecular Weight [Da]
34	Natural Substrates/ Products (Substrates)
3	Organic Solvent Stability
236	Organism
7	Pathway
116	PDB ID
24	pH Optimum
2	pH Range
2	pH Stability
1	pI Value
2	Posttranslational Modification
156	Protein Variants
32	Purification (Commentary)
2	Reaction
3	Reaction Type
164	Reference
4	Renatured (Commentary)
7	Source Tissue
26	Specific Activity [micromol/min/mg]
4	Storage Stability
197	Substrates and Products (Substrate)
54	Subunits
170	Synonyms
1	Systematic Name
26	Temperature Optimum [°C]
4	Temperature Range [°C]
37	Temperature Stability [°C]
224	Turnover Number [1/s]

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Turnover Number on EC 1.1.1.85 - 3-isopropylmalate dehydrogenase

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0025 - 220

(2R,3S)-3-isopropylmalate

84 entries

0.016 - 0.85

3-(2'-methylthio)ethylmalate

6 entries

38

3-ethylmalate

Acidithiobacillus ferrooxidans

-

-

639161

20

3-isoamylmalate

Acidithiobacillus ferrooxidans

-

-

639161

3.8 - 14.9

3-isopropylmalate

12 entries

28

3-methylmalate

Acidithiobacillus ferrooxidans

-

-

639161

2.7

3-tert-butylmalate

Acidithiobacillus ferrooxidans

-

-

639161

3.1

D-3-ethylmalate

Sulfurisphaera tokodaii

P50455

pH 8.0

680429

3.1

D-3-isobutylmalate

Sulfurisphaera tokodaii

P50455

pH 8.0

680429

2.7

D-3-isopentylmalate

Sulfurisphaera tokodaii

P50455

pH 8.0

680429

3.6

D-3-isopropylmalate

Sulfurisphaera tokodaii

P50455

pH 8.0

680429

3.5

D-3-methylmalate

Sulfurisphaera tokodaii

P50455

pH 8.0

680429

0.96

D-3-t-butylmalate

Sulfurisphaera tokodaii

P50455

pH 8.0

680429

0.41

D-malate

Escherichia coli

-

pH 7.5, 37°C

763032

70

ethylmalate

Escherichia coli

-

pH 7.6

639164

0.26

homoisocitrate

Sulfolobus acidocaldarius

Q4JB37

at pH 8.0 and 60°C

738195

0.09 - 69

isopropylmalate

13 entries

0.19 - 10.6

malate

6 entries

0.15 - 220

NAD+

78 entries

0.09 - 4.69

NADP+

6 entries

0.1 - 0.26

Tartrate

4 entries

6

tert-butylmalate

Escherichia coli

-

pH 7.6

639164

additional information

additional information

Thermus thermophilus

-

the cold adaption results from the destabilization of the ternary complex caused by increase in the volume of the residue at position 126

657223

-

0.0025

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q5SIY4

mutant enzyme D241A, at pH 7.6 and 20°C

737729

0.0025

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q5SIY4

mutant enzyme K185A, at pH 7.6 and 20°C

737729

0.003

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme D292N, at pH 7.5 and 25°C

738685

0.017

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme Y181A, at pH 7.5 and 25°C

738685

0.023

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme R146K, at pH 7.5 and 25°C

738685

0.03

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme R146A, at pH 7.5 and 25°C

738685

0.033

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q5SIY4

mutant enzyme E270A, at pH 7.6 and 20°C

737729

0.033

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q5SIY4

mutant enzyme E270A, in the presence of K+, at pH 7.6 and 20°C

738239

0.033

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme R136K, at pH 7.5 and 25°C

738685

0.045

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q5SIY4

mutant enzyme D217A, at pH 7.6 and 20°C

737729

0.08

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q72IW9

wild-type HICDH, pH 8.0, 37°C

711150

0.108

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q5SIY4

mutant enzyme Y139A, at pH 7.6 and 20°C

737729

0.233

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme Y181F, at pH 7.5 and 25°C

738685

0.3

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q72IW9

mutant E57V/S72I/R85M/Y86A/M208T/V238M/R310M, pH 8.0, 37°C

711150

0.4

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q72IW9

mutant R85V/Y86A, pH 8.0, 37°C

711150

0.433

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q5SIY4

mutant enzyme D245A, at pH 7.6 and 20°C

737729

0.47

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme L132A, at pH 7.5 and 25°C

738685

0.55

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q5SIY4

mutant enzyme N102A, at pH 7.6 and 20°C

737729

0.583

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme Y181H, at pH 7.5 and 25°C

738685

0.6

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q72IW9

mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M, pH 8.0, 37°C

711150

0.62

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

mutant L133F, pH not specified in the publication, temperature not specified in the publication

725437

0.622

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

wild-type, pH not specified in the publication, temperature not specified in the publication

725437

0.73

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

mutant L134F, pH not specified in the publication, temperature not specified in the publication

725437

1.2

(2R,3S)-3-isopropylmalate

synthetic construct

-

acIPMDH-ML, pH 8.0, 25°C

763696

1.6

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q72IW9

mutant S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C

711150

1.62

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme R174K, at pH 7.5 and 25°C

738685

1.7

(2R,3S)-3-isopropylmalate

synthetic construct

-

ancIPMDH-IQ, pH 8.0, 25°C

763696

1.8

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q72IW9

mutant E57V/S72I/R85M/Y86A/M208T/F217Y/R310M, pH 8.0, 37°C

711150

2

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme V235A, at pH 7.5 and 25°C

738685

2.36

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 8.0, 40°C, wild-type enzyme

639156

2.41

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 8.0, 40°C, mutant enzyme C275T

639156

2.9

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q72IW9

mutant H15Y/E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C

711150

2.9

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 7.6, 20°C, quenched flow experiment

724976

3.1

(2R,3S)-3-isopropylmalate

Shewanella oneidensis

-

pressure 250 MPa, 20°C, pH not specified in the publication

711511

3.1

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

mutant enzyme L133A, at pH 7.5 and 25°C

738685

3.4

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 7.6, 20°C, stopped flow experiment

724976

3.83

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

mutant F137L, pH not specified in the publication, temperature not specified in the publication

725437

3.97

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q5SIY4

wild type enzyme, in the presence of K+, at pH 7.6 and 20°C

738239

4.3

(2R,3S)-3-isopropylmalate

synthetic construct

-

acIPMDH-ML, pH 8.0, 40°C

763696

4.57

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93882

wild type enzyme, at pH 7.5 and 25°C

738685

4.6

(2R,3S)-3-isopropylmalate

synthetic construct

-

ancIPMDH-IQ, pH 8.0, 40°C

763696

5.1

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q72IW9

mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C

711150

5.7

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q72IW9

wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C

711150

5.8

(2R,3S)-3-isopropylmalate

Shewanella oneidensis

-

pressure 200 MPa, 20°C, pH not specified in the publication

711511

6.22

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

wild-type, pH not specified in the publication, temperature not specified in the publication

725437

7.2

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 8.0, 40°C, mutant enzyme A31G/G43A/A709G

639156

7.6

(2R,3S)-3-isopropylmalate

Shewanella oneidensis

-

pressure 100 MPa, 20°C, pH not specified in the publication

711511

8.4

(2R,3S)-3-isopropylmalate

Shewanella oneidensis

-

pressure 150 MPa, 20°C, pH not specified in the publication

711511

8.5

(2R,3S)-3-isopropylmalate

Sulfolobus acidocaldarius

Q4JB37

at pH 8.0 and 60°C

738195

9.05

(2R,3S)-3-isopropylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

wild-type, pH not specified in the publication, temperature not specified in the publication

725437

9.6

(2R,3S)-3-isopropylmalate

Shewanella benthica

-

mutant enzyme I268A, at pH 7.6 and 25°C

738191

10

(2R,3S)-3-isopropylmalate

Shewanella benthica

D2YZL2

pressure 250 MPa, 20°C, pH not specified in the publication

711511

10.7

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 8.0, 40°C, mutant enzyme G43A

639156

12

(2R,3S)-3-isopropylmalate

Escherichia coli

-

pH 7.5, 37°C

763032

13

(2R,3S)-3-isopropylmalate

Shewanella benthica

D2YZL2

pressure 200 MPa, 20°C, pH not specified in the publication

711511

14

(2R,3S)-3-isopropylmalate

Shewanella oneidensis

-

pressure 0.1 MPa, 20°C, pH not specified in the publication

711511

14

(2R,3S)-3-isopropylmalate

Shewanella benthica

D2YZL2

pressure 100 MPa, 20°C, pH not specified in the publication

711511

14

(2R,3S)-3-isopropylmalate

Shewanella benthica

D2YZL2

pressure 150 MPa, 20°C, pH not specified in the publication

711511

14.8

(2R,3S)-3-isopropylmalate

Shewanella benthica

-

mutant enzyme M106L, at pH 7.6 and 25°C

738191

15

(2R,3S)-3-isopropylmalate

Shewanella benthica

D2YZL2

pressure 0.1 MPa, 20°C, pH not specified in the publication

711511

15

(2R,3S)-3-isopropylmalate

Shewanella oneidensis

-

pressure 50 MPa, 20°C, pH not specified in the publication

711511

16.1

(2R,3S)-3-isopropylmalate

Shewanella oneidensis

Q8E9N3

mutant enzyme A268I, at pH 7.6 and 25°C

738191

16.4

(2R,3S)-3-isopropylmalate

Shewanella benthica

-

wild type enzyme, at pH 7.6 and 25°C

738191

16.7

(2R,3S)-3-isopropylmalate

Shewanella oneidensis

Q8E9N3

mutant enzyme S266A, at pH 7.6 and 25°C

738191

17

(2R,3S)-3-isopropylmalate

Shewanella benthica

D2YZL2

pressure 50 MPa, 20°C, pH not specified in the publication

711511

17.4

(2R,3S)-3-isopropylmalate

Shewanella oneidensis

Q8E9N3

wild type enzyme, at pH 7.6 and 25°C

738191

17.7

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 8.0, 40°C, mutant enzyme G376A

639156

20.4

(2R,3S)-3-isopropylmalate

Shewanella benthica

-

mutant enzyme A266S, at pH 7.6 and 25°C

738191

22

(2R,3S)-3-isopropylmalate

synthetic construct

-

acIPMDH-ML, pH 8.0, 70°C

763696

22

(2R,3S)-3-isopropylmalate

synthetic construct

-

ancIPMDH-IQ, pH 8.0, 70°C

763696

28

(2R,3S)-3-isopropylmalate

Acidithiobacillus ferrooxidans

-

-

639161

40

(2R,3S)-3-isopropylmalate

Thermus thermophilus

Q5SIY4

wild type enzyme, at pH 7.6 and 20°C

737729

44.7

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 8.0, 70°C, mutant enzyme C275T

639156

45.8

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 8.0, 70°C, wild-type enzyme

639156

48.1

(2R,3S)-3-isopropylmalate

Shewanella oneidensis

Q8E9N3

mutant enzyme L106M, at pH 7.6 and 25°C

738191

50.1

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 8.0, 70°C, mutant enzyme A31G/G43A/A709G

639156

63.6

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 8.0, 70°C, mutant enzyme G43A

639156

100

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

pH 8.0, 70°C, mutant enzyme G376A

639156

100

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

55°C, mutant enzyme A172D

639158

107

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

55°C, wild-type enzyme

639158

111

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

55°C, mutant enzyme A172Q

639158

117

(2R,3S)-3-isopropylmalate

Thermus thermophilus

-

55°C, mutant enzyme A172E and A172N

639158

163

(2R,3S)-3-isopropylmalate

Thermus thermophilus

P61494

60°C, pH 7.6, wild-type enzyme

748065

220

(2R,3S)-3-isopropylmalate

Thermus thermophilus

P61494

60°C, pH 7.6, mutant enzyme A172V

748065

0.016

3-(2'-methylthio)ethylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

wild-type, pH not specified in the publication, temperature not specified in the publication

725437

0.0167

3-(2'-methylthio)ethylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

wild-type, pH not specified in the publication, temperature not specified in the publication

725437

0.033

3-(2'-methylthio)ethylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

mutant F137L, pH not specified in the publication, temperature not specified in the publication

725437

0.27

3-(2'-methylthio)ethylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

mutant L134F, pH not specified in the publication, temperature not specified in the publication

725437

0.367

3-(2'-methylthio)ethylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

mutant L133F, pH not specified in the publication, temperature not specified in the publication

725437

0.85

3-(2'-methylthio)ethylmalate

Arabidopsis thaliana

P93832, Q9FMT1, Q9SA14

wild-type, pH not specified in the publication, temperature not specified in the publication

725437

3.8

3-isopropylmalate

Sulfurisphaera tokodaii

P50455

pH 7.5, 70°C, recombinant mutant F3S/S232G

701046

6

3-isopropylmalate

Sulfurisphaera tokodaii

P50455

pH 7.5, 70°C, recombinant mutant R284G

701046

6.8

3-isopropylmalate

Sulfurisphaera tokodaii

P50455

pH 7.5, 70°C, recombinant mutant F3S

701046

6.9

3-isopropylmalate

Sulfurisphaera tokodaii

P50455

pH 7.5, 70°C, recombinant wild-type enzyme

701046

7.5

3-isopropylmalate

Sulfurisphaera tokodaii

P50455

pH 7.5, 70°C, recombinant mutant L288P

701046

7.8

3-isopropylmalate

Sulfurisphaera tokodaii

P50455

pH 7.5, 70°C, recombinant mutant F3S/L288P

701046

8.3

3-isopropylmalate

Bacillus subtilis

-

mutant Glu253Trp

687442

9.5

3-isopropylmalate

Bacillus subtilis

-

mutant Glu253Ala

687442

9.9

3-isopropylmalate

Bacillus subtilis

-

mutant Glu253Gln

687442

12.4

3-isopropylmalate

Bacillus subtilis

-

mutant Glu253Val

687442

13.7

3-isopropylmalate

Bacillus subtilis

-

mutant Glu253Leu

687442

14.9

3-isopropylmalate

Bacillus subtilis

-

wild-type

687442

0.09

isopropylmalate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NADP+, mutant enzyme S226R/D278K/I279Y

639159

0.15

isopropylmalate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, wild-type enzyme

639159

0.2

isopropylmalate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G

639155

0.21

isopropylmalate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q

639155

0.24

isopropylmalate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, wild-type enzyme

639155

0.26

isopropylmalate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NADP+, wild-type enzyme

639159

0.48

isopropylmalate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, mutant enzyme S226R

639159

0.88

isopropylmalate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NADP+, mutant enzyme S226R

639159

1.66

isopropylmalate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NADP+, wild-type enzyme

639155

1.91

isopropylmalate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, mutant enzyme S226R/D278K/I279Y

639159

8

isopropylmalate

Thermus thermophilus

-

pH 7.6, 70°C

639164

19

isopropylmalate

Thermus thermophilus

-

pH 7.6, 40°C

639164

69

isopropylmalate

Escherichia coli

-

pH 7.6, 40°C

639164

0.19

malate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q

639155

0.26

malate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G

639155

4

malate

Sulfurisphaera tokodaii

P50455

-

680429

4.69

malate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NADP+, wild-type enzyme

639155

5

malate

Acidithiobacillus ferrooxidans

-

-

639161

10.6

malate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, wild-type enzyme

639155

0.15

NAD+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme

639159

0.19

NAD+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with tartrate, wild-type enzyme or reaction with malate, mutant enzyme E87Q

639155

0.2

NAD+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87G

639155

0.21

NAD+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with tartrate, mutant enzyme E87G

639155

0.21

NAD+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87Q

639155

0.24

NAD+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme

639155

0.26

NAD+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with malate, mutant enzyme E87G or reaction with tartrate, mutant enzyme E87Q

639155

0.37

NAD+

Thermus thermophilus

Q5SIY4

pH 8.0, 25°C, wild-type enzyme

763739

0.48

NAD+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R

639159

1.2

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant V126A

657223

1.2

NAD+

synthetic construct

-

acIPMDH-ML, pH 8.0, 25°C

763696

1.5

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant V126S

657223

1.7

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant V126G

657223

1.7

NAD+

synthetic construct

-

ancIPMDH-IQ, pH 8.0, 25°C

763696

1.9

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant V126T

657223

1.91

NAD+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R/D278K/I279Y

639159

2 - 3.7

NAD+

Shewanella benthica

-

mutant enzyme A266S, at pH 7.6 and 25°C

738191

2.36

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, wild-type enzyme

639156

2.4

NAD+

Thermus thermophilus

Q5SIY4

pH 8.0, 40°C, wild-type enzyme

763739

2.4

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, wild-type

657223

2.41

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant enzyme C275T

639156

3.5

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant V126E

657223

4.3

NAD+

synthetic construct

-

acIPMDH-ML, pH 8.0, 40°C

763696

4.6

NAD+

synthetic construct

-

ancIPMDH-IQ, pH 8.0, 40°C

763696

6

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant V126I

657223

7.2

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant enzyme A31G/G43A/A709G

639156

8

NAD+

Thermus thermophilus

-

pH 7.6, 70°C, reaction with isopropylmalate

639164

8.5

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant V126L

657223

9

NAD+

Thermus thermophilus

-

pH 8.0, 60°C, mutant V126A

657223

10

NAD+

Thermus thermophilus

-

pH 8.0, 60°C, V126G

657223

10.2

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutant V181T

668635

10.3

NAD+

Shewanella benthica

-

mutant enzyme I268A, at pH 7.6 and 25°C

738191

10.6

NAD+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with malate, wild-type enzyme

639155

10.7

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant enzyme G43A

639156

11

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant V126F

657223

11.2

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutant D184H

668635

13

NAD+

Thermus thermophilus

-

pH 8.0, 60°C, mutant V126S

657223

13.2

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutant V181T/P324T/A335E

668635

13.4

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant wild-type enzyme

668635

13.8

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutant P324T

668635

14.6

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutant P56E

668635

15

NAD+

Thermus thermophilus

-

pH 8.0, 60°C, mutant V126T

657223

15

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutants F53L and V61I

668635

15.2

NAD+

Shewanella benthica

-

mutant enzyme M106L, at pH 7.6 and 25°C

738191

15.6

NAD+

Shewanella oneidensis

Q8E9N3

mutant enzyme S266A, at pH 7.6 and 25°C

738191

17.1

NAD+

Shewanella oneidensis

Q8E9N3

wild type enzyme, at pH 7.6 and 25°C

738191

17.4

NAD+

Shewanella oneidensis

Q8E9N3

mutant enzyme A268I, at pH 7.6 and 25°C

738191

17.7

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant enzyme G376A

639156

17.7

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutant R58L

668635

18

NAD+

Thermus thermophilus

-

pH 8.0, 40°C, mutant V126M

657223

18.2

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutants S261N and A335E

668635

18.7

NAD+

Shewanella benthica

-

wild type enzyme, at pH 7.6 and 25°C

738191

19

NAD+

Thermus thermophilus

-

pH 7.6, 40°C, reaction with isopropylmalate

639164

20

NAD+

Thermus thermophilus

-

pH 8.0, 60°C, wild-type

657223

21

NAD+

Thermus thermophilus

-

pH 8.0, 60°C, mutant V126E

657223

21.3

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutant H197K

668635

22

NAD+

synthetic construct

-

acIPMDH-ML, pH 8.0, 70°C

763696

22

NAD+

synthetic construct

-

ancIPMDH-IQ, pH 8.0, 70°C

763696

25.2

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutant L134N/V181T/P324T/A335E

668635

29.9

NAD+

Thermus thermophilus

Q5SIY4

pH 7.6, 70°C, recombinant mutant L134N

668635

30.5

NAD+

Thermus thermophilus

-

pH 8.0, 60°C, mutant V126I

657223

42

NAD+

Thermus thermophilus

-

pH 8.0, 60°C, mutant V126F

657223

44.5

NAD+

Thermus thermophilus

-

pH 8.0, 60°C, mutant V126L

657223

44.7

NAD+

Thermus thermophilus

-

pH 8.0, 70°C, mutant enzyme C275T

639156

45.8

NAD+

Thermus thermophilus

-

pH 8.0, 70°C, wild-type enzyme

639156

50.1

NAD+

Thermus thermophilus

-

pH 8.0, 70°C, mutant enzyme A31G/G43A/A709G

639156

50.5

NAD+

Shewanella oneidensis

Q8E9N3

mutant enzyme L106M, at pH 7.6 and 25°C

738191

54

NAD+

Thermus thermophilus

-

pH 8.0, 60°C, mutant V126M

657223

63.6

NAD+

Thermus thermophilus

-

pH 8.0, 70°C, mutant enzyme G43A

639156

69

NAD+

Escherichia coli

-

pH 7.6, 40°C, reaction with isopropylmalate

639164

79

NAD+

Thermus thermophilus

Q5SIY4

pH 8.0, 70°C, wild-type enzyme

763739

100

NAD+

Thermus thermophilus

-

pH 8.0, 70°C, mutant enzyme G376A

639156

100

NAD+

Thermus thermophilus

-

55°C, mutant enzyme A172D

639158

107

NAD+

Thermus thermophilus

-

55°C, wild-type enzyme

639158

111

NAD+

Thermus thermophilus

-

55°C, mutant enzyme A172Q

639158

117

NAD+

Thermus thermophilus

-

55°C, mutant enzyme A172E and A172N

639158

163

NAD+

Thermus thermophilus

P61494

60°C, pH 7.6, wild-type enzyme

748065

220

NAD+

Thermus thermophilus

P61494

60°C, pH 7.6, pH 7.6, mutant enzyme A172V

748065

0.09

NADP+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R/D278K/I279Y

639159

0.1

NADP+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with tartrate, wild-type enzyme

639155

0.26

NADP+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme

639159

0.88

NADP+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R

639159

1.66

NADP+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme

639155

4.69

NADP+

Thermus thermophilus

-

pH 7.3, 21°C, reaction with malate, wild-type enzyme

639155

0.1

Tartrate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NADP+, wild-type enzyme

639155

0.19

Tartrate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, wild-type enzyme

639155

0.21

Tartrate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G

639155

0.26

Tartrate

Thermus thermophilus

-

pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q

639155