1.1.1.85: 3-isopropylmalate dehydrogenase

This is an abbreviated version, for detailed information about 3-isopropylmalate dehydrogenase, go to the full flat file.

Reaction

(2R,3S)-3-isopropylmalate
+
NAD+
=
(2S)-2-isopropyl-3-oxosuccinate
+
NADH
+
H+

Synonyms

2-hydroxy-4-methyl-3-carboxyvalerate:NAD+ oxidoreductase, 2R,3S-isopropylmalate:NAD+ oxidoreductase (decaboxylating), 3-IPM dehydrogenase, 3-IPM-DH, 3-isopropylmalate dehydrogenase, 3-isopropylmalateDH, APS IPMDH, beta-IPM dehydrogenase, beta-IPMDH, beta-isopropylmalate dehydrogenase, beta-isopropylmalic enzyme, Bs3-isopropylmalateDH, dehydrogenase, 3-isopropylmalate, IMDH, IPMDH, IPMDH1, IPMDH2, IPMDH3, isopropylmalate dehydrogenase, leuB, NAD-dependent isopropylmalate dehydrogenase, Saci_0600, SbIPMDH, SoIPMDH, threo-Ds-3-isopropylmalate dehydrogenase, Tt-beta-3-isopropylmalateDH, two-domain 3-isopropylmalate dehydrogenase, Ydr417cp

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.85 3-isopropylmalate dehydrogenase

Temperature Stability

Temperature Stability on EC 1.1.1.85 - 3-isopropylmalate dehydrogenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
pH 8.3, half-life: 5 min
23
-
pH 8.3, half-life: 5 min, essentially full protection against inactivation is provided by either 20% v/v glycerol or by simultaneous presence of Mn2+ and dithiothreitol, Mn2+ and beta-isopropylmalate or Mn2+ and alpha-ketoisocaproate
50
-
thermal stability curves of wild-type and mutant enzymes at pH 7.6 in presence of 0.5 mM EDTA, overview
60
-
5 min, 92% loss of activity in absence of NaCl, 6% loss of activity in presence of 1.5 M NaCl
61
-
midpoint of thermal unfolding curve, mutant enzyme A172D
63
-
midpoint of irreversible denaturation
65
-
midpoint of thermal unfolding curve, mutant enzyme A172V
66
-
midpoint of thermal unfolding curve, mutant enzyme A172I and A172E
67
-
midpoint of thermal unfolding curve, mutant enzyme A172L and A172F
70
-
10 min, 40% loss of activity
79
-
midpoint of irreversible denaturation
82
-
10 min, 50% inactivation, mutant enzyme A31G/G43A/A709G
83
-
10 min, 50% inactivation
85 - 95
-
thermal degeneration curves of wild-type and mutant enzymes at pH 7.6, overview
85
-
10 min, 50% inactivation, mutant enzyme G43A
97
-
melting temperature; pH 7.0, 50% inactivation; Tm-value
additional information