1.1.1.85: 3-isopropylmalate dehydrogenase

This is an abbreviated version, for detailed information about 3-isopropylmalate dehydrogenase, go to the full flat file.

Reaction

(2R,3S)-3-isopropylmalate
+
NAD+
=
(2S)-2-isopropyl-3-oxosuccinate
+
NADH
+
H+

Synonyms

2-hydroxy-4-methyl-3-carboxyvalerate:NAD+ oxidoreductase, 2R,3S-isopropylmalate:NAD+ oxidoreductase (decaboxylating), 3-IPM dehydrogenase, 3-IPM-DH, 3-isopropylmalate dehydrogenase, 3-isopropylmalateDH, APS IPMDH, beta-IPM dehydrogenase, beta-IPMDH, beta-isopropylmalate dehydrogenase, beta-isopropylmalic enzyme, Bs3-isopropylmalateDH, dehydrogenase, 3-isopropylmalate, IMDH, IPMDH, IPMDH1, IPMDH2, IPMDH3, isopropylmalate dehydrogenase, leuB, NAD-dependent isopropylmalate dehydrogenase, Saci_0600, SbIPMDH, SoIPMDH, threo-Ds-3-isopropylmalate dehydrogenase, Tt-beta-3-isopropylmalateDH, two-domain 3-isopropylmalate dehydrogenase, Ydr417cp

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.85 3-isopropylmalate dehydrogenase

Purification

Purification on EC 1.1.1.85 - 3-isopropylmalate dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DE52 column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
gene cloned in Escherichia coli
-
Ni Sepharose 6 column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
overexpression in Escherichia coli
-
purified by heat treatment followed by nickel affinity chromatography
-
recombinant enzyme
-
recombinant enzyme expressed in Escherichia coli
recombinant wild-type and mutant enzymes from Escherichia coli strain MA153 by anion exchange and hydrophobic interaction chromatography
recombinant wild-type and mutants from Escherichia coli strain MA153 by anion exchange and adsorption chromatography
-
using a HisTrap HP column
using Ni-NTA chromatography
-