1.1.1.85: 3-isopropylmalate dehydrogenase

This is an abbreviated version, for detailed information about 3-isopropylmalate dehydrogenase, go to the full flat file.

Reaction

(2R,3S)-3-isopropylmalate
+
NAD+
=
(2S)-2-isopropyl-3-oxosuccinate
+
NADH
+
H+

Synonyms

2-hydroxy-4-methyl-3-carboxyvalerate:NAD+ oxidoreductase, 2R,3S-isopropylmalate:NAD+ oxidoreductase (decaboxylating), 3-IPM dehydrogenase, 3-IPM-DH, 3-isopropylmalate dehydrogenase, 3-isopropylmalateDH, APS IPMDH, beta-IPM dehydrogenase, beta-IPMDH, beta-isopropylmalate dehydrogenase, beta-isopropylmalic enzyme, Bs3-isopropylmalateDH, dehydrogenase, 3-isopropylmalate, IMDH, IPMDH, IPMDH1, IPMDH2, IPMDH3, isopropylmalate dehydrogenase, leuB, NAD-dependent isopropylmalate dehydrogenase, Saci_0600, SbIPMDH, SoIPMDH, threo-Ds-3-isopropylmalate dehydrogenase, Tt-beta-3-isopropylmalateDH, two-domain 3-isopropylmalate dehydrogenase, Ydr417cp

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.85 3-isopropylmalate dehydrogenase

General Stability

General Stability on EC 1.1.1.85 - 3-isopropylmalate dehydrogenase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
complete loss of activity in presence of 4 M urea
-
dilute solutions, 1 mg/ml or less, precipitate upon thawing
-
in contrast to 3-isopropylmalate dehydrogenase from other sources, K+ and Na+ are not essential for stability of the Sulfolobus enzyme
-
K+ does not stabilize the enzyme
-
slow inactivation occurs in presence of relatively high concentrations of EDTA, 0.01 M
-