1.1.1.83: D-malate dehydrogenase (decarboxylating)

This is an abbreviated version, for detailed information about D-malate dehydrogenase (decarboxylating), go to the full flat file.

Reaction

(R)-malate
+
NAD+
=
pyruvate
+
CO2
+
NADH

Synonyms

D-malate dehydrogenase, D-malic enzyme, dehydrogenase, D-malate (decarboxylating), dmlA

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.83 D-malate dehydrogenase (decarboxylating)

Application

Application on EC 1.1.1.83 - D-malate dehydrogenase (decarboxylating)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
sequential fluorometric quantification of malic acid enantiomers in a single line flow-injection system using immobilized-enzyme reactors. An immobilized D-malate dehydrogenase (EC 1.1.1.83) reactor and an immobilized L-malate dehydrogenase (EC 1.1.1.40) reactor are introduced into the flowline in series. Sample and coenzyme (NAD+ or NADP+) are injected into the flow line by an open sandwich method. D-Malate is selectively oxidized by EC 1.1.1.83 when NAD+ is injected with a sample. When NADP+ is injected with a sample, L -malate is oxidized only by 1.1.1.40. NADH or NADPH produced by the immobilized-enzyme reactors is monitored fluorometrically at 455 nm